Background & Aims of the Study: This study aimed to investigate, for the first time, the ability of single-cell oil production from low-cost carbon sources using Kocuria Y205 native Iranian bacterial isolates from the soil.Materials and Methods: Whey and lignocellulose compounds were used as carbon sources and yeast extract as a nitrogen source. Also, the isolated cultivation was done on Mineral Salts Medium (MSM) culture media. Molecular analysis based on 16s rRNA gene sequence was performed to identify isolated. Fourier-Transform Infrared Spectroscopy (FTIR) analysis was used to confirm the presence of carbon groups. GC analysis was also used to identify the fatty acids Sudan black. Finally, a Transmission Electron Microscopy (TEM) electron microscopy image was taken to view the stored lipid granule. Results:The highest rate of lipid production among all carbon sources in different periods of cultivation by whey was 24.57% after 48 hours, and also, the highest rate of lipid production for lignocellulose compounds was 15.29% after 48 hours. Conclusion:This study shows that the newly isolated Kocuria Y 205 has excellent ability to use whey and lignocellulose compounds as waste containing carbon resources for the growth, production, and storage of microbial oil. It can be used in industrial applications, too.