Extracts from culture filtrates of Gibberella fujikuroi grown under various fermentation conditions have yielded the following metabolites : 7-hydroxy-,

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Phlorizin, a potent inhibitor of the Na+ /D‐glucose cotransporter, was derivatised to 3‐aminophlorizin and subsequently coupled to Affi‐Gel 15. Affinity chromatography of pig kidney brush border membranes solubilised in Triton X‐100 allowed the purification of a 60 kDa protein on this resin. We consider this protein to be the Na+ /D‐glucose cotransporter, or part of it, for the following reasons: (i) binding of this protein to Affi‐Gel 15 specifically requires phlorizin covalently attached to the resin and is lowered when phlorizin is replaced by phloretin; (ii) binding of the 60 kDa protein to a phlorizin affinity column requires the presence of Na+; (iii) polyclonal as well as monoclonal antibodies against the 60 kDa protein inhibit binding of phlorizin to brush border membranes from rabbit and pig kidney.