This study evaluated three fermented milk beverages to which had been added sugar strawberry puree post‐fermentation. The base was composed of 70% of milk, with whey and buttermilk in the concentrations of 30% and 0%, 15% and 15%, and 0% and 30%, respectively. The starter culture developed well with all formulations reaching pH 4.7–4.9 in 180 min of fermentation. Lactic acid bacteria in the products were above 8 log cfu/mL throughout the study. The beverages presented similar pH, acidity and viscosity. Buttermilk and whey can be interesting ingredients to be added into fermented milk beverages, because the consumers liked all the products equally, which had an average acceptance score exceeding ‘liked moderately’.

The aim of this work was to verify the viability of Lactobacillus casei in chocolate flan as well as its survival under simulated human gastrointestinal conditions. After 1 and 15 days of manufacture, the dessert was evaluated for L. casei population and subsequently submitted to acidic solution for 30, 60, 90 and 120 min and to bile salts solution for 150 min. After each time, L. casei population was evaluated. The results were evaluated by variance analysis (ANOVA) and Tukey's test. The population of L. casei in the flan was above 10 9 CFU/g during the shelf life, whose value is above the limit established for a probiotic food by the Brazilian legislation. L. casei was relatively resistant to the simulated gastrointestinal conditions tested in this study, once the population reduced during the in vitro tests but remained above 10 6 CFU/g. Chocolate flan showed to be an excellent food for addition of probiotic microorganisms, especially L. casei, once it was observed high populations in the product even after in vitro resistance tests simulating the human digestive process. Key words: Dairy desserts, probiotic microorganisms, shelf life ResumoNeste trabalho, verificou-se a viabilidade de Lactobacillus casei em flan de chocolate, além de sua sobrevivência em condições que simulam o trato gastrointestinal humano. Após 1 e 15 dias da produção, a população de L. casei presente na sobremesa foi avaliada, e o flan foi submetido, em seguida, a uma solução ácida, durante 30, 60, 90 e 120 minutos, e a uma solução de sais biliares, por um período de 150 minutos. Após cada um desses tempos, a população de L. casei foi observada. Os resultados foram avaliados por meio de análise de variância (ANOVA) e teste de Tukey. A população de L. casei no flan foi maior que 10 9 UFC/g durante a vida de prateleira do produto, valor este, maior que o estabelecido para um alimento probiótico pela legislação brasileira. L. casei mostrou-se relativamente resistente às condições simuladas do trato gastrointestinal avaliadas neste trabalho, uma vez que apesar de sua população ter sido reduzida durante os testes in vitro, esta manteve-se acima de 10 6 UFC/g. O flan de chocolate mostrou-se um excelente alimento para a introdução de microrganismos probióticos, especialmente L. casei, uma vez que observou-se altas populações no produto, mesmo após os testes de resistência in vitro simulando parte do processo digestivo humano. Palavras-chave: Microrganismos probióticos, sobremesas lácteas, vida de prateleira

This study aimed to compare the microbiological and physicochemical quality of raw milk from individual and collective tanks, identify the psychrotrophic microbiota of refrigerated raw milk and verify compliance with current legislation. The enumeration of proteolytic and lipolytic psychrotrophs, as well as, populations of mesophilic aerobes, total coliforms, E. coli and enterobacteria was carried out. The psychrotrophic microbiota was characterized by morpho-tinctorial tests. Determination of fat content, protein, lactose, total solids, titratable acidity, cryoscopic index and somatic cells count (SCC) were also conducted. The average counts of mesophilic aerobes did not met the minimum quality requirements; however, the average results of SCC and the physicochemical parameters were in accordance with Brazilian legislation. The psychrotrophs counts were on average 90% of the total count of mesophilic aerobes, with psychrotrophic population of less than 6 log CFU/ml. A high percentage of proteolytic and lipolytic psychrotrophs in relation to psychrotrophs total counts was found. Considering the two weeks testing, there was a significant difference (P> 0.05) between milk samples from collective and individual tanks only for total coliforms and for protein and lactose contents. Although the predominant psychrotrophic microbiota was Gram negative bacilli, but Gram positive bacteria were also found. Thus, the population of mesophilic aerobes in disagreement with the legislation and the high counts of psychrotrophic and Gram negative population in milk are indicative that yet, there are problems in the sanitary-hygienic production, storage and transportation of refrigerated raw milk produced in the region studied. Therefore, there is a gap between the practices recommended by legislation and the actions really found in the Brazilian milk production chain. ResumoEste estudo teve como objetivo comparar a qualidade microbiológica e físico-química de leite cru proveniente de tanques individuais e coletivos, identificar a microbiota psicrotrófica do leite cru refrigerado e verificar o cumprimento da legislação vigente para este alimento. Realizou-se a contagem da microbiota de psicrotróficos, proteolíticos, lipolíticos, aeróbios mesófilos, coliformes totais, E. coli e enterobactérias. A microbiota psicrotrófica foi caracterizada através de testes morfotintoriais. Análises dos teores de gordura, proteína, lactose, sólidos totais, acidez titulável, índice crioscópico e contagem de células somáticas (CCS) também foram realizados. As contagens médias de aeróbios mesófilos não atenderam aos requisitos mínimos de qualidade, já os resultados médios de CCS e os parâmetros físico-químicos estudados estavam de acordo com a legislação vigente. A contagem de psicrotróficos foi, em média, 90% da contagem total de aeróbios mesófilos, com contagens de psicrotróficos menores que 6 log UFC/ml. Foi encontrada alta porcentagem de proteolíticos e lipolíticos em relação à contagem total de psicrotróficos. Considerando as duas semanas...