Amyloid fibril formation is a shared property of all proteins; therefore, model proteins can be used to study this process. We measured protein aggregation of the model amyloid-forming protein stefin B in the presence and absence of several antioxidants. Amyloid fibril formation by stefin B was routinely induced at pH 5 and 10% TFE, at room temperature. The effects of antioxidants NAC, vitamin C, vitamin E, and the three polyphenols resveratrol, quercetin, and curcumin on the kinetics of fibril formation were followed using ThT fluorescence. Concomitantly, the morphology and amount of the aggregates and fibrils were checked by transmission electron microscopy (TEM). The concentration of the antioxidants was varied, and it was observed that different modes of action apply at low or high concentrations relative to the binding constant. In order to obtain more insight into the possible mode of binding, docking of NAC, vitamin C, and all three polyphenols was done to the monomeric form of stefin B.

Introduction:Cysteine protease are biological catalysts which play a pivotal role in numerous biological reactions in organism. Much of the literature is inscribed to their biochemical significance, distribution and mechanism of action. Many diseases, e.g. Alzheimer’s disease, develop due to enzyme balance disruption. Understanding of cysteine protease’s disbalance is therefor a key to unravel the new possibilities of treatment. Cysteine protease are one of the most important enzymes for protein disruption during programmed cell death. Whether protein disruption is part of cell deaths is not enough clear in any cases. Thereafter, any tissue disruption, including proteolysis, generate more or less inflammation appearance.Review:This review briefly summarizes the current knowledge about pathological mechanism’s that results in AD, with significant reference to the role of cysteine protease in it. Based on the summary, new pharmacological approach and development of novel potent drugs with selective toxicity targeting cysteine protease will be a major challenge in years to come.

The paper explores the existence of cognitive linguistics principles in translation of emotion-related metaphorical expressions. Cognitive linguists (Lakoff & Johnson, 1980;Lakoff, 1987) define metaphor as a mechanism used for understanding one conceptual domain, target domain, in terms of another conceptual domain, source domain, through sets of correspondences between these two domains. They also claim that metaphor is omnipresent in ordinary discourse. Cognitive linguists, however, also realized that certain metaphors can be recognized and identified in different languages and cultures whereas some are language-and culture-specific. This paper focuses on similarities and variations in metaphors which have recently become popular within the discipline of Translation Studies. Transferring and translating metaphors from one language to another can represent a challenge for translators due to a multi-faceted process of translation including both linguistic and non-linguistic elements. A number of methods and procedures have been developed to overcome potential difficulties in translating metaphorical expressions, with the most frequent ones being substitution, paraphrase, or deletion. The analysis shows the transformation of metaphorical expressions from one language into another and the procedures involving underlying conceptual metaphors, native speaker competence, and the influence of the source language.

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