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RESUMO: "Atividades leishmanicida, bactericida e antioxidante do extrato hidroetanólico das folhas de Caryocar brasiliense Cambess". As atividades antimicrobiana e antioxidante do extrato hidroetanólico das folhas de Caryocar brasiliense foram estudadas. O extrato demonstrou efeito leishmanicida sobre formas promastigotas de Leishmania amazonensis e atividade bactericida sobre estirpes de bactérias patogênicas para o homem. Além disso, o extrato demonstrou relevante capacidade antioxidante, similar às atividades da vitamina C e da rutina. Unitermos:Caryocar brasiliense, Caryocaraceae, piqui, atividade leishmanicida, atividade antioxidante, DPPH, leishmaniose. ABSTRACT:The antimicrobial and antioxidant activities of the hydroethanolic extract from Caryocar brasiliense leaves were evaluated. The extract showed leishmanicidal effect against Leishmania amazonensis promastigote forms and bactericidal activity against some pathogenic bacteria. The extract also showed relevant antioxidant activity, similar to that of vitamin C and rutin.

Several essential oil samples from dried flower-heads of

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BackgroundDengue virus infection is a public health threat to hundreds of millions of individuals in the tropical regions of the globe. Although Dengue infection usually manifests itself in its mildest, though often debilitating clinical form, dengue fever, life-threatening complications commonly arise in the form of hemorrhagic shock and encephalitis. The etiological basis for the virus-induced pathology in general, and the different clinical manifestations in particular, are not well understood. We reasoned that a detailed knowledge of the global biological processes affected by virus entry into a cell might help shed new light on this long-standing problem.MethodsA bacterial two-hybrid screen using DENV2 structural proteins as bait was performed, and the results were used to feed a manually curated, global dengue-human protein interaction network. Gene ontology and pathway enrichment, along with network topology and microarray meta-analysis, were used to generate hypothesis regarding dengue disease biology.ResultsCombining bioinformatic tools with two-hybrid technology, we screened human cDNA libraries to catalogue proteins physically interacting with the DENV2 virus structural proteins, Env, cap and PrM. We identified 31 interacting human proteins representing distinct biological processes that are closely related to the major clinical diagnostic feature of dengue infection: haemostatic imbalance. In addition, we found dengue-binding human proteins involved with additional key aspects, previously described as fundamental for virus entry into cells and the innate immune response to infection. Construction of a DENV2-human global protein interaction network revealed interesting biological properties suggested by simple network topology analysis.ConclusionsOur experimental strategy revealed that dengue structural proteins interact with human protein targets involved in the maintenance of blood coagulation and innate anti-viral response processes, and predicts that the interaction of dengue proteins with a proposed human protein interaction network produces a modified biological outcome that may be behind the hallmark pathologies of dengue infection.