Breast cancer is one of the most common types of cancer in Saudi Arabia, as well as worldwide. Most breast cancer cases are detected at advanced stages, and one possible reason is that many Saudi women are not aware of breast self-examination (BSE). Few studies have examined the knowledge and practice of BSE among female nursing students in Saudi Arabia, and most of those have focused on major cities. Therefore, the aim of this study is to determine the knowledge and practice of BSE among Saudi nursing students at Majma’ah University, Kingdom of Saudi Arabia. This study used a descriptive cross-sectional design involving 100 nursing students there. The results of this research have revealed that there is a low level of practice of BSE among nursing students and irregular performance of BSE for those who practice it. Subsequently, there is an urgent need to empower awareness, attitude, and practice of BSE among nursing students in Saudi Arabia.