Background: Hepatitis B virus (HBV) infection is a serious public health problem in sub-Saharan Africa. The study aimed to determine the sero prevalence of HCV among HBV patients. Methods: This was a cross-sectional study conducted at Military Hospital-Omdurman. Data including socio-demographical information, age, residence, gender, tribes) also history of hospitalization and blood transfusion, were collected using a structured questionnaire. Blood specimen were collected for detection of antibodies to hepatitis C virus using indirect-Elisa method ,Ethical clearance and informed consent were obtained prior to the enrolment in the study. Data were analyzed using the SPSS program. Results: Elisa test showed that only 4% of patients in this study showed positive result. Most of participants (60%) aged between 30-40 years, followed by 30% between 40-50 years, 10% between 20-30 years of age, 30% of patients were hospitalized while 70% were not hospitalized. Conclusions: Low percentage of HBV patients showed positive ELISA test result of HCV. Severe acute hepatitis B was more frequent in the HBV-HCV co infected group than in the HBV infected group.