The pandemic flu (2009) is a global outbreak of a new strain of H1N1 influenza virus that referred as "swine flu". The 2009 flu pandemic vaccines (H1N1 vaccines) are the biological preparation of influenza vaccines that have been developed to protect and improve immunity against the pandemic H1N1/09 virus. Squalene has been added to vaccine as immunologic adjuvant to stimulate the immune system, increase the response to the vaccine and reduce amount of usage vaccine. Thimerosal has been added to some vaccines as a preservative because it is effective in preventing bacterial contamination, particularly in multidose containers. To investigate the possible immunological effects of swine flu vaccine, Albino rats were injected with H1N1 antigen contained thimerosal, adjuvant and H1N1 vaccine. Results obtained in the present study showed that squalene and thimerosal contributed to the regiment of adverse reactions and symptoms, which act as detergents instead of its helper role of vaccine that undergone toxicity effectors in the biological pathways including; immunopathological and histopathological effects; the damage was done by vaccine, particularly when potentiated by powerful "immunoenhancers" caused a strong immunostimulation that emphasized by results of this research as well as its biological hazards which are more pronounced when act together as whole vaccine than they act individually so the adverse events that resulted from treatment with squalene-adjuvanted vaccine are more potent than those of either antigen or squalene-treated groups.