The importance of this study is that it is deals with the Barada River pollution with hydrocarbons (crude oil and its derivatives), as well as evaluating the ability of different types of Micrococcus sp. to bioremediation of hydrocarbons. Ten strains of Micrococcus sp. were isolated from the water samples from Barada River in Damascus, and these isolated were classified depending on Bergey's manual to four species: M. luteus, M. lylae, M. flavus, and M. antarcticus. The crude oil dependent growths of these isolates were assessed for 15 days at 30°C by monitoring the gradient fluxes in pH, optical density OD and total viable count TVC in the medium. The result was statistically supported by applying the One-way ANOVA test and the Paired sample t-test. The results showed that activity of species was best at the concentration of 2% of crude oil, and the species of M. lylae was best in dealing with crude oil, although this superiority between species was not supported at the statistical studying. In general, the different species of Micrococcus sp. have a good ability to deal with the different concentrations (1, 2 and 3%) of crude oil. And this supports research which encourages the use of this genus in the bioremediation. This research was indicated to the ability of M. lylae to deal with different concentrations of crude oil, which was not mentioned in previous research KEYWORDS