The present study evaluated the total phenolic and flavonoid content as well as the antioxidant activity of methanolic leaf extracts of five plants from Brazilian flora: Abarema cochliacarpos, Croton corchoropsis, Myroxylon peruiferum, Stryphnodendron pulcherrimum and Tanaecium cyrtanthum by 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and total antioxidant capacity assays. A thin layer chromatography analysis of all plant extracts has also been performed and it showed the presence of different types of secondary metabolites, namely saponins, phenylpropanoids and flavonoids. Among the studied plants, A. cochliacarpos and S. pulcherrimum showed considerable antioxidant radical scavenging activity on all the tested assays and they also exhibited substantial amounts of phenolic compounds. In addition, a positive correlation was found between total phenols and both ABTS radical scavenging activity and total antioxidant capacity assays, thus indicating the major role of phenols on the antioxidant activity of these plants. To the best of the authors' knowledge, this is the first approach where the phenolic content and antioxidant activity of A. cochliacarpos, C. corchoropsis, M. peruiferum, S. pulcherrimum and T. xanthophyllum were explored.