Background: Translation of Tyr codons is highly prone to Phe misincorporation during amino acid limitation in CHO cells. Results: CHO TyrRS is error-prone and readily aminoacylates tRNA Tyr with Phe. Conclusion: Mammalian TyrRS has evolved to be significantly less accurate than its bacterial counterpart. Significance: Different evolutionary constraints determine the accuracy of translation quality control in eukaryotes and bacteria.
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