Nursing team professionals are the health professionals most exposed to Occupational Accidents with exposure to Biological Material (ATMB) in the hospital environment. The most frequent type is related to sharps, followed by contamination by contact of the skin and mucosa with blood, fluids and/or secretions. ATMBs represent a global public health problem, both because of the frequency with which they occur and the severity of the repercussions on the health of professionals. The Regulatory Norm 32 recommends basic guidelines for the implementation of measures to protect the safety and health of health service workers, but studies show low or partial adherence to these guidelines. Objective:To develop and evaluate an educational video to instruct the nursing team on NR 32 -Biological Risks Method: This is a methodological study, for the construction and validation of an educational video. Contextualized Instructional Design was used, which is composed of five stages: analysis, design, development, implementation and evaluation. In the Analysis stage, data from the Work Accident Reports (CATs) of nursing professionals at the institution where the study was developed were collected, the video script was designed in the design, the animation format, the actual video was elaborated using the Cognitive Theory of Multimedia Learning (TCAM) by Richard Mayer (2009) as a reference. In the Implementation phase, the video was made available on the Google Drive platform so that the material could be evaluated first. The evaluation stage involved the validation of the video content, firstly, by expert judges in the field of occupational health and health professionals, and later by the target audience. Results: An 80% increase in CAT records with nurses was observed when comparing the period before the pandemic (2019) and during the pandemic (2020). Of the accidents analyzed, 80% were percutaneous, 17% involved mucosal and 3% cutaneous. A minimum Content Validation Index (CVI) value of 80% was considered acceptable for content validation. Thus, the video was considered valid by the expert judges with an overall CVI of 91% and by the target audience with a general CVI of 100%. Conclusions: The educational video "NR 32 your safety in the first place" proved to be valid both by the judges and by the target audience, with the potential to contribute to educational actions in a hospital context, being able to integrate programs to promote the health of the worker and encourage the adherence of the nursing team to the recommendations and guidelines of NR 32.