The fabrication ionic magnetic core-shell nanoparticles were simple synthesize with a super-ferromagnetic and small particle size properties, which enabled sufficient DNA particle loading with easy isolation based on an external magnetic field.

The objective of this study was to complete the molecular evaluation of five Acacia species including first by determining the genetic diversity of the plants using the polymerase chain reaction (PCR)-based inter-simple sequence repeat (ISSR) method. This investigation was carried out to assess fingerprint and thus genetic variations among the Acacia species. The ISSR method was used to determine DNA fingerprints for Acacia spp. Eight primers were used, with all primers delivering amplification products. Our data show a total of 71 bands of 70 bp to 2,200 bp were amplified, of which 0.77 demonstrated an average polymorphism information content per primer. Among the eight primers tested, the mean annealing temperature was 48°C and average polymorphism information content was between 0.36 and 0.84. The ISSR primers for the five species of Acacia showed four main groups, with a higher level of similarity between these species. These results indicated ISSR markers provide an efficient alternate for identification via DNA fingerprinting of the genetic relationships in Acacia. PCR-based ISSR represents a powerful method that can provide practical information for the development of molecular markers, molecular cytogenetic techniques, and DNA Fingerprinting for application in an Acacia spp breeding program.

New design of cationic magnetic core–shell nanoparticles fabricated with a large hydrophilic group (carbohydrate molecules) enabled high adsorption of a nucleic acid, easy isolation and controlled the movement by applying an external magnetic field.

Background Forensic DNA phenotyping has gained momentum in the recent past due to the prediction of externally visible characters (EVCs) from the biological sample. The most common phenotypes like eye, hair, and skin color are predicted from the biological samples using a web-based system called IrisPlex. Based on six genetic SNPs, the IrisPlex system is developed and validated for its prediction accuracy in diverse ethnic groups worldwide. In previous studies, this system proved to have significant prediction accuracy. The EVCs vary substantially based on different geographical locations. Hence, the objective of this study was to validate the accuracy of the IrisPlex system in predicting the eye colors in the Iraqi population. Methods Six genetic single-nucleotide polymorphisms SNPs (HERC2-rs12913832, OCA2- rs1800407, SLC24A4-rs12896399, SLC45A2- rs16891982, TYR-rs1393350, and IRF4- rs12203592) in 58 Iraqi subjects were performed using Sequenom MassARRAY Genotyping. According to Liu et al., a predicted probability of 0.7 was considered as the threshold. Results Participants in this study of brown color were observed in 44.83%, intermediate in 43.1%, and blue in 12.07%. Completely predictive accuracy is obtained in 1; we observed the AUC at threshold 0.7 was 0.91 for brown, 0.79 for blue, and 0.60 for intermediate eye color. The sensitivity was 42.85% for blue, 0% for intermediate eye color, and 100% for brown-colored eye. Specificity was 100% for blue, 100% for intermediate, and 78.13% for brown eye color. Conclusion Hence, it was concluded that the prediction accuracy of the IrisPlex system for blue and brown color eye in the Iraqi population is significant in the studied population size. However, a pivotal study with larger sample size is required to represent the prediction accuracy of the IrisPlex system in the whole Iraqi population.

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