The objectives of this study: Prevalence and in-silico analysis of lipid and cardiac enzymes in ACS patients. A total of 213 (137 male and 76 female) blood samples were taken from cardiac patients. After prevalence analysis was done on blood samples of patients & to check the biomarker level of all cardiac and lipid enzymes in all patients to find out the acute coronary syndrome. Statical analysis was done using the spss 21 software and In-silico analysis was also done in this study. In statical analysis, correlation showed a relationship with lipid, cardiac, walk and exercise with significant level P<0.05, 0.01. Smoking and walk have a positive relationship with cardiac and lipid enzymes show a negative relationship with smoking and exercise with sig. level p< 0.05. The regression analysis has shown CPK, C.K.M.B, LDH, V.L.D.L. positively and significantly predicted with smoking. Cholesterol, Triglyceride, AST negatively and significantly predicted with smoking which shows that F has 17.602% and R esquire has a .339% variation with smoking.  Insilco analysis shows that Selenocysteine, Asparagine, and Lysine are binding residues involved in TSP1-human and TIQ ligand in molecular docking. ligand and protein, have -5.7 is the lowest energy and distance between is 3.137 to 5.829. More exercise could reduce ACS problems in cardiac patients. New markers and therapies are discovered in the future for biotechnologies.

Cotton fiber genes and promoters are of great importance in understanding fiber development mechanism as well as for improvement of fiber. Sucrose phosphate synthase gene (SPS) (insert abbreviation of gene)has found to express at higher rate in developing cotton fibers. It is an important enzyme that have major role in sucrose as well as cellulose synthesis. Upstream region of a SPS gene from cotton was retrieved through HTGS database and analyzed using bioinformatics tools. Sequence analysis identified various regulatory motifs including light, drought, heat responsiveness and MYB binding sites in the promoter. The SPS promoter was isolated from cotton genomic DNA (using what method) and fused to β-glucuronidase (GUS) gene in a plant expression vector. Transient GUS expression analysis in various cotton tissues showed that promoter was active in the fiber tissues. Full length 2 kb SPS promoter showed high expression in fibers during elongation and secondary cell wall synthesis stage. A 1.5 kb deletion fragment showed reduced expression in fibers. Our results suggest that cotton SPS promoter may be used to express genes specifically in fiber cells for improvement of cotton fiber quality traits.