The radio-over-fiber (RoF) technique has gained a lot of interest recently, as the millimeter-wave signals can be generated and delivered in the optical domain with the advantages of low attenuation, high capacity, and being free from electromagnetic noise interference (EMI). In this paper, we propose and experimentally prove a self-start multi-wavelength laser source based on a distributed feedback laser diode (DFB-LD) for the RoF transport system. The self-start multi-wavelength laser source generates stable laser power with less than 0.18 dB power fluctuation and exhibits good stability. In order to estimate the transmission performance, data is externally modulated onto the multi-wavelength by a reflective semiconductor optical amplifier (RSOA) and transmitted through single-mode fiber (SMF). The experimental result proves that the proposed RoF transport system achieves error-free transmission and clear eye diagrams.