Batrachochytrium dendrobatidis is a fungus belonging to the Phylum Chytridiomycota, Class Chytridiomycetes, Order Chytridiales, and is the highly infectious aetiological agent responsible for a potentially fatal disease, chytridiomycosis, which is currently decimating many of the world's amphibian populations. The fungus infects 2 amphibian orders (Anura and Caudata), 14 families and at least 200 species and is responsible for at least 1 species extinction. Whilst the origin of the agent and routes of transmission are being debated, it has been recognised that successful management of the disease will require effective sampling regimes and detection assays. We have developed a range of unique sampling protocols together with diagnostic assays for the detection of B. dendrobatidis in both living and deceased tadpoles and adults. Here, we formally present our data and discuss them in respect to assay sensitivity, specificity, repeatability and reproducibility. We suggest that compliance with the recommended protocols will avoid the generation of spurious results, thereby providing the international scientific and regulatory community with a set of validated procedures which will assist in the successful management of chytridiomycosis in the future.

A survey confi rmed the presence of the amphibian chytrid fungus, Batrachochytrium dendrobatidis in a number of frog habitats close to major cities and towns across Tasmania.The detection of chytrid infection in some remote wetlands at high altitude locations on the Tas manian Central Plateau is of particular concern. The likely presence of chytrid infections was assessed by examining tadpoles for signs of depigmentation, thinning and asymmetry in their keratinized jaw sheaths using a hand lens. Assessing the jaw sheaths of up to 60 tadpoles combined with the application of the Taqman chytrid PCR test was a useful means of detecting the presence of ch yt ridiomycosis at each frog habitat.