The quality sound of singing birds strongly depends on testosterone. Unfortunately, most of the testosterone will be converted to estradiol by the aromatase enzyme. The study aimed to examine the potential of natural aromatase blockers for increasing the frequency and quality of canary chirps. Five adult male canaries, with bodyweight 25-30 g were used in the study. After being adapted for a week, birds were fed a mixture of aromatase blockers and canary seed for 20 days at a dose of 0.3 mg /30 g body weight. Quantities of a song using Hagemon touch methods were calculated by frequency of chirping for 10 minutes, whereas the quality of the song was counted by the periods of once chirping for 10 minutes. Recording begins when the bird is given standard feeding and repeated after treatment for 20 days. The results showed that the frequency of chirping before given a mixture of ingredients was 15.3 /10 min, after being treated with aromatase blockers the frequency increased up to 78.9 /10 min. The length of the tweet before treatment was <1s while after treatment up to 17s. It can be concluded that aromatase blockers can increase the frequency and quality of male Canary's song.
The response of estrus can be enhanced by providing PGF2α and a combination of PGF2αGnRH so that the time of mating using Artificial Insemination (AI) can be held precisely. This study aims to compare the response of estrus synchronization using PGF2α compare to PGF2α-GnRH. Twenty female cattle Ongole Crossbreds, 4-6 years, BCS average of 3-4 were used in this research. Cattle were selected based on their luteal phase at the days of 7 to 18 by rectal examination. Then, all of the cattle were injected by cloprostenol® with a dose of 500 ug intrauterine. Ten of the animals were injected by 2.5 mL of Fertagyl® intramuscularly after 48 hours then continued observing and response estrus detection. The onset of estrus using PGF2α was detected on 75.94 ± 0.78, while 41.57 ± 28.40 hours for PGF2α-GnRH Using statistical, there was a significant difference (P<0.05). Duration of estrus phase using PGF2α was 24.65 ± 0.49 whereas PGF2α-GnRH was 10.88 ± 7.45 hours. Using statistical methods, there was a significant difference (P <0.05). It could be concluded that the use of PGF2α was more effective for the duration and intensity of estrus, and a combination of PGF2α -GnRH is more effective for stimulating estrus.
The effects of pregnancy and lactation on hematochemical and mineral parameters were evaluated in Ettawa cross-bred goats in Disctrict Kulonprogo, Yogyakarta, Indonesia. Blood plasma samples were collected from 14 th pregnant goats (2 month n=4; 3 month n=6; 4 month n=4) and 16 lactation goats (1 month, n= 4; 2 month, n=5; 3 month, n=3 and 4 month, n=4). Blood serum samples were collected from 18 pregnanct goats (1 month, n=3; 2 month, n=2; 3 month, n=6; 4 month, n=4) and 16 lactation goats (1 month, n=4; 2 month, n=5; 3 month, n=3; 4 month, n=4). The does averaged 2.5-4 years old and their mean body weights were 30-40 kg. The current result showed that pregnant does recorded higher (p<0.05) values of RBCs and hematocrites compared to lactating does. The higher RBCs during the first and third month of pregnantion and the higher hematocrites values during the first of pregnantion. There were not significant differences in MCV, MCH, MCHC, neutrophyls, limphocytes, monocytes, WBCs, eosinophyls, and minerals Mg2+, in organic , Na+, K+ and Cl-during pregnantion and lactation. The results indicated that pregnantion and lactation has significant effect on RBCs and hematocrites of Ettawa crossbred goats in district Disctrict Kulonprogo, Yogyakarta, Indonesia
Progesterone (P4) is a dominant hormone during pregnancy. In the later stage of pregnancy, the stress hormone particularly cortisol (C) may increase for initiating the parturition process as a consequence of fetal stress. This study was a preliminary study to compare the concentration of P4 and C in feces of Etawah Crossbreed Goat during their last stage of pregnancy and post partus. This study used 5 pregnant Etawah Crossbreed Goats (t 20th weeks) of pregnancy. Fecal samples were collected in the 20th week of pregnancy to 2 weeks of postpartum. All fecal samples were then dried using a freeze dryer (Labfreez FD10-MR) for 7 days at -80°C. Afterward, dried feces were pulverized and extracted by using 3ml of methanol 80%. The fecal extract was then analyzed the P4 and C concentrations using the enzyme-linked immunosorbent assay method. Concentrations of P4 and C metabolites in the last stage of pregnancy were 5,506.18 3,396.72 ng/g dry feces and 136,625.83 42,479.22 ng/g feces, respectively. Concentrations of P4 and C metabolites in the 2 weeks postpartum decreased at 669.38 P 643.9 ng/g feces and 110,295 / 14,378, 8 ng/g feces, respectively. It canbe concluded that there was a difference in the fecal progesterone and cortisol concentrations between the last phase of pregnancy and the postpartum phase.
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