Whey protein was digested with one of seven kinds of proteases at 37 degrees C (trypsin, proteinase K, actinase E, thermolysin, or papain) or at 25 degrees C (pepsin or chymotrypsin) for 24 h. The digested samples were assayed for the inhibitory activity of angiotensin-converting enzyme and for changes in the systolic blood pressure caused in spontaneously hypertensive rats after gastric intubation. The strongest depressive effect on the systolic blood pressure (-55 mm Hg) was observed at 6 h after gastric intubation of the whey protein that was digested by proteinase K. Finally, six peptides were chromatographically isolated from the proteinase K digest by a combination of hydrophobic reversed-phase HPLC and gel filtration. The amino acid sequences and their origins were clarified as follows: Val-Tyr-Pro-Phe-Pro-Gly [beta-casein (CN); f 59-64], Gly-Lys-Pro (beta 2-microglobulin; f 18-20), Ile-Pro-Ala (beta-lactoglobulin; f 78-80), Phe-Pro (serum albumin; f 221-222; beta-CN, f 62-63, f 157-158, and f 205-206), Val-Tyr-Pro (beta-CN; f 59-61), and Thr-Pro-Val-Val-Val-Pro-Pro-Phe-Leu-Gln-Pro (beta-CN; f 80-90). Chemical synthesis of these six peptides confirmed that all peptides, except an undecapeptide, have antihypertensive activity in spontaneously hypertensive rats. The synthetic tripeptide Ile-Pro-Ala, originating from beta-lactoglobulin, showed the strongest antihypertensive activity (-31 mm Hg).

Civet coffee has been known as the most expensive coffee in the world. Its high prices are mainly contributed by its uncommon production method. Civet coffee is produced from the coffee beans which have been digested by Asian palm civet or Luwak (Paradoxorus hermaprodites). Civet coffee production is very limited due to its processing is merely depends on civet biologic system. Unfortunately, reliable information on civet microbial digestive is still limited. The aim of this research is to evaluate cup (sensory) and microbial quality of wild and caged civet coffee. Samples of wild and caged civet coffee obtained from Bener Meriah District, Aceh, Indonesia. Sensory quality parameters consist of fragrance, aroma, flavour, aftertaste, acidity, body, balance, uniformity, clean cups, sweetness and overall. Microbial quality was done by enumeration of lactic acid bacteria. The results showed that wild and caged civet coffee relatively had similar profile sensory and microbial characteristics. The score cupping of wild civet coffee is slightly higher than caged civet coffee. Aroma and flavour characteristics of wild and caged civet coffee were herby, nutty, grassy tobacco-like, fishy and sweet but there were over fruity-papaya and guava-like aroma and flavour of caged civet coffee. The lactic acid bacteria count on wild and caged civet were 5.8 x 10 8 cfu/ml and 3,8 x10 8 cfu/ml. Three different colonies from MRS media were isolated and submitted to gram and catalase test. All isolates showed gram-positive and catalase negative. The result confirmed that most colonies are lactic acid bacteria group. Two isolates (ICF 1 and ICF 3) showed proteolytic activity, and isolated ICF 1 showed the largest zone of clearance of 15 mm. Further investigation is required for identification and selection of performance lactic acid bacteria for artificial civet coffee fermentation.

Kopi luwak, is an Indonesian exotic coffee, is known as one of most popular coffee in the world. This coffee is prepared from the finest and ripest coffee berries that are eaten by Asian civet (Paradoxurus hermaphroditus), cat like-animal. The aim of this study was to determine the chemical attributes of kopi luwak both in green and roasted coffee bean. This study conducted by collecting the six samples of kopi luwak from different farms in Gayo Highland, which then processed onto green and roasted coffee beans. The examined parameters are protein, lipid, caffeine and chlorogenic acids. The results of this study showed that the differences existed in chemical attributes of green and roasted of Gayo kopi luwak. The average contents of lipid, protein, caffeine and chlorogenic acids of Gayo arabica kopi luwak in green bean were 12.30%, 13.36%, 1.20% and 3.73%, while in roasted bean were 14.79%, 13.66%, 1.10% and 0.88% respectively. To be conclude with, lipids and caffeine survived during thermal treatments in roasting process, meanwhile chlorogenic acids and protein (N-compunds) are degraded and formed smaller fragments with lower molecular weights.

Buku ini kami susun untuk membantu pemahaman masyarakat umum maupun mahasiswa mengenai kopi luwak dan karakteristiknya. Sejauh ini buku yang mengulas kopi luwak sangat langka. Dengan latar belakang sejarah keberadaan kopi luwak yang belum genap seabad dikenal oleh masyarakat dunia, keterbatasan informasi baik teori maupun praktis tentu menjadi kendala dalam menyusunnya menjadi sebuah buku yang sempurna.

The use of turmeric as an active compound for preservatives is widely used because it is simple, inexpensive, and easy to find. To prevent the losses of quality and damage to the meat, processing and preservation is required. The purpose of this study was to determine the effect of using different percentages of turmeric flour on the pH and cooking losses of chicken meat. This study used 2.2 kg Broiler chicken breast and 214.5 g turmeric flour. The research design used was a completely randomized design consisting of 4 treatments and 5 replications so that the total sample was 20 units. With the percentage of using turmeric flour T0 (0%), T1 (10%), T2 (13%) and T3 (16%). The results of research that have been carried out the percentage of using turmeric flour as much as 13% (5.90) is the best treatment for the pH of broiler chicken meat. The results of research that have been carried out the percentage of use of turmeric flour as much as 13% (25.35%) is the best treatment for cooking losses.