Riparian zones along rivers and streams provide ecosystem services that may change over time as disturbances increase and deteriorate these buffer zones globally. The effect of stressors on ecosystem services along the rivers in underdeveloped countries is unclear, which impacts the environment directly in the form of riparian health indicators (RHIs). This study fills this gap and measures the impact of stressors on RHIs (parameters of habitat, plant cover, regeneration, exotics, and erosion) in the Indus River basin (IRB) in Pakistan. Data on 11 stressors and 27 RHIs were collected using a field-based approach in 269 transects in the upper and lower Indus basins (UIB and LIB) in 2020 and analyzed using multivariate statistical methods. The Kruskal–Wallis tests (p < 0.05) indicated that RHIs varied significantly under the influence of stressors in the UIB and LIB. However, their highest mean values were found in the UIB. Principal component analysis revealed the key RHIs and stressors, which explained 62.50% and 77.10% of the variance, respectively. The Pearson correlation showed that stressors had greater impacts on RHIs in LIB (with r ranging from −0.42 to 0.56). Our results also showed that stressors affected RHI indices with r ranging from −0.39 to 0.50 (on habitat), −0.36 to 0.46 (on plant cover), −0.34 to 0.35 (on regeneration), −0.34 to 0.56 (on erosion), and −0.42 to 0.23 (on exotics). Furthermore, it was confirmed by the agglomerative hierarchical cluster that indices and sub-indices of RHIs and stressors differ across the UIB and LIB. These findings may serve as guidance for managers of large rivers and ecosystem service providers to minimize the environmental impact of stressors in terms of RHIs.

Riparian buffers and stream channel widths along river networks have extremely significant ecological influences on parameters and stressors associated with riparian health indicators (RHIs). It is imperative for countries that rely heavily on rivers for irrigation to protect RHIs such as habitat, plant cover, regeneration, exotics, and erosion. It is unclear which protection methods are most effective for RHIs in less developed countries, such as Pakistan. This study fills this gap by using a quick field-based technique that includes 273 transects and examines the response of RHIs in the upper and lower Indus River basins (IRB). In the lower Indus basin (LIB), riparian buffer and stream channel widths had the most considerable influence on RHIs using Pearson’s correlations, ranging from ̶ 0.47 &lt; r &lt; 0.71 and ̶ 0.41 &lt; r &lt; 0.32, respectively. There was a significant relationship between stressors and RHIs in the LIB when these widths were changed, and stressors had a significant influence on habitat ̶ 0.37 &lt; r &lt; 0.41, plant cover ̶ 0.32 &lt; r &lt; 0.38, regeneration ̶ 0.29 &lt; r &lt; 0.25, erosion ̶ 0.34 &lt; r &lt; 0.49, and exotics ̶ 0.39 &lt; r &lt; 0.24. In contrast, these stressors in the upper Indus basin (UIB) also adversely affected habitat ̶ 0.28 &lt; r &lt; 0.27, plant cover ̶ 0.34 &lt; r &lt; 0.26, regeneration ̶ 0.19 &lt; r &lt; 0.26, erosion ̶ 0.38 &lt; r &lt; 0.23, and exotics ̶ 0.31 &lt; r &lt; 0.30. It was found from the principal component analysis that the responses of RHIs and stressors varied considerably between the UIB and LIB. Additionally, the agglomerative hierarchical cluster analysis of the RHIs and stressor indices revealed dissimilarities in the UIB and LIB. This study supports the need to examine riparian regions along long rivers, which are subject to the same administrative strategies. Large river ecosystems need revised standards to prevent further degradation based on ecological indicators.