The current research aimed to use the Random Amplification Polymorphic DNA (RAPD) markers based on the polymerase chain reaction (PCR) technique to find genetic variation between six cultivars of  Prunus armeniaca L. and Prunus domistica L planted in North Iraq. DNA was extracted from young plant leaves, Thirteen primers were used in RAPD reactions, and samples were migrated using electrophoresis using an agarose gel. The results showed that the concentration of the extracted DNA ranged between (25.8 - 66.3) μg/ml, and the results showed a discrepancy in the number and molecular size of the DNA bands for each cultivar, as the total number of bands reached (1038), of which 882 were polymorphic, and 156 monomorphic bands.