The study aimed to detect molecular intraspecific markers to identify seven cultivars of Kalanchoe blossfeldina Poeln. (K. blossfeldiana Calandiva Bardot; K. blossfeldiana Calandiva Hayworth; K. blossfeldiana Calandiva Monro; K. blossfeldiana Calandiva Fonda; K. blossfeldiana Calandiva Middler; K. blossfeldiana Calandiva Leonardo; K. blossfeldiana Calandiva Rosalina) have been genetically analyzed, using Randomly Amplified Polymorphic DNA (RAPD PCR) technique, ten randomize primers used for this purpose yielded 390 reproducible bands with an average of 39 bands or fragments per primer, contain 259 monomorphic bands and 145 were polymorphic bands, primers produce 12 unique bands and 8 bands were absent. The primers (80-14) yielded high number of DNA fragments (53). The results showed 48 polymorphic sites and 32 monomorphic sites, the heist polymorphism percentage reached 100% with primer (OPB-01) and the lowest polymorphism percentage with the primer (80-15) reached 25%, UPGMA analysis depending on similarity matrix, studied cultivars showed the highest genetic similarity reached (0.908) between (Calandiva Monro, Calandiva Fonda), while lowest genetic distance was (0.426) between (Calandiva Leonardo, Calandiva Hayworth) cultivars as well as genetic difference was (0.347) between (Calandiva Leonardo, Calandiva Hayworth) also. Phylogenetic dendrogram showed two main clusters, the first cluster composed of two secondary clusters comprised of 6 cultivars, while the second main cluster contain only one cultivar (Calandiva Leonardo) which showed the highest molecular differences and the purple flowers .