Cleaning In Place (CIP) system of cleaning the interior surface of pipelines, vessels, filters, process equipment and associated things without dismantling. Depending on the processing practice and load of soiling on the process equipment, the cleaning solutions may be used for single cycle or recycled and reused for multi use. Cleaning-In-Place systems shortens the required time for cleaning, besides using detergents and disinfectants at higher concentrations and temperatures, as well as the recovery of the cleaning solutions. The automation of these systems also allows for safe and reproducible results and economic optimization of the process. In CIP system, cleaning solutions used include various types of detergents, sanitizers or disinfectants. To achieve the most effective CIP results, it is necessary to design the production process and the CIP components and circuits simultaneously, giving equal consideration to production and cleaning requirements
The objective of the current study was to identify the effect of reusing the alkali solution on thermoduric count in pasteurized milk. The study was carried out in a commercial Dairy Plant, where reusing of alkali solution is not practiced. In the present study, 28 samples each were collected from raw milk, pasteurized milk, and alkali solution before and after its use. Equivalent numbers of samples from surface swab of storage tank were also collected after cleaning procedure. Immediately after collection samples were plated for evaluating Total Viable Count (TVC) and thermoduric count on plate count agar. The genera which comes under the thermoduric bacteria are characterized based on colony morphology, Gram staining, biochemical reactions and 16s rDNA PCR. The result indicated that the isolated organism was B. megaterium. The isolated organisms were subjected to acid, alkali and heat adaptation studies.
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