In the experiment to determine the plasma electron temperature, a modified multichannel PIN diodes assembly is used as detectors to record the X-ray pulses from a low-energy Mather-type plasma focus device energized by a 32 pF, 15 kV (3.6 kJ) single capacitor, with deuterium as a filling gas. The ratio of the integrated bremsstrahlung emission transmitting through foils to the total incident flux as a function of foil thickness at various temperatures is obtained for foil absorbers of material. Using 3 pm, 6 pm, 9 pm,12 pm,13 pm and 18 pm thick aluminium absorbers, the transmitted X-ray flux is detected. By comparing the experimental and theoretical curves through a computer program, the plasma electron temperature is determined. Results show that the deuterium focus plasma electron temperature is about 800 eV.

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ABSTRACT: Background: Failure of treatment with antibiotics occurs due to increase in number of Multidrug resistant gram negative bacteria, worldwide. The objective of this study was to find out the antimicrobial activity of crude ethanolic extract and its further three fractions by Ocimum basilicum leaves against multi drug resistant gram negative rods. Material and Methods: This descriptive study was conducted in the Department of Microbiology, University of Health Sciences, Lahore from 1st july 2016 to 30th june 2017. Total 80 multidrug resistant gram negative rods were included in this study. Agar dilution method was performed to determine MIC of crude ethanolic extract and different fractions i-e n-hexane, chloroform and ethyl acetate of Ocimum basilicum leaves against multidrug resistant gram negative rods i-e ESBLs and carbapenemase producers. Muti-inoculater was used for inoculation. Results: The mean MICs of crude ethanolic extract, n-hexane fraction, chloroform fraction, and ethyl acetate fraction of Ocimum basilicum against ESBLs were 100.0±8.00, 168.13±8.00, 176.88±8.00 41.75±8.00 respectively. Similarly, the mean MICs of crude ethanolic extract, n-hexane fraction, chloroform fraction, and ethyl acetate fraction of Ocimum basilicum against carbapenemase producers were 77.50±8.00, 113.75±8.00, 132.50±8.00 and 29.50±8.00 respectively. Conclusions: Ethyl acetate fraction and crude ethanolic extract from leaves of Ocimum basilicum showed good antibacterial effectiveness against ESBLs and carbapenem resistant organisms than other fractions. This finding may also promote the effective use of O. basilicum herb and its components in modern medicine.