No abstract

Microscopic preparations of renal tumours from 76 nephrectomized patients were reviewed retrospectively, to establish a possible dependence of the prognosis on various histological criteria. The preparations were twice reviewed "blind". The three criteria, cell type, necrosis, and structure, were found to be reproducible from the first to the second examination, but this was not the case with invasion. Of the three parameters, only cell type and necrosis were significant for the prognosis, tumows without necrosis and composed of clear cells having the best prognosis, while tumours composed of granular cells had the poorest prognosis. If there was more than one section from a tumour, there was often great variance in morphology from section to section. This finding has not been studied statistically in the present investigation. Scand J Urol Nephrol Downloaded from informahealthcare.com by QUT Queensland University of Tech on 11/01/14 For personal use only. Scand J Urol Nephrol Downloaded from informahealthcare.com by QUT Queensland University of Tech on 11/01/14 For personal use only. Scand J Urol Nephrd 8 Scand J Urol Nephrol Downloaded from informahealthcare.com by QUT Queensland University of Tech on 11/01/14 For personal use only.

No abstract

The measurement of lactate dehydrogenase (LDH) release into perfusates after hypothermic storage was found to be a reliable index of ischemic injury of rabbit kidneys. Kidneys were exposed to warm and cold ischemia for varying periods. Each kidney was perfused before and after storage at simple hypothermia with 25 ml of a modified Collins solution. The venous effuent was collected in 5 ml fractions. Total LDH activity was measured in the first fraction after storage and used as a measure of ischemic tissue damage. It was confirmed that increasing the period of cold ischemia result in significant increases in LDH activity. The release of LDH into perfusates was then used to compare kidney damage after preservation with various fluids. With this method, it was not possible to demonstrate any difference in the extent of tissue damage after preservation with sodium-rich vs. potassium-rich perfusion fluid. Addition of steroids, vitamins and essential amino acids did not prevent or reduce tissue damage, estimated in this way. The effects of adding cryoprotectants to the perfusion fluid varied; LDH release following addition of 5% DMSO was significantly greater, and after addition of 5% glycerol smaller than the release after perfusion with a modified Collins solution alone. Stepwise addition of DMSO up to 20% resulted in serious tissue damage with a large LDH release into the perfusate.