Artemisia sect. Tridentatae is composed of 11 species of xerophytic shrubs, which dominate much of western North America. Sequences of the internal transcribed spacers (ITS) of nuclear ribosomal DNA were used to construct a phylogeny, examine circumscription of the section, resolve interspecific relationships, and test competing hypotheses on the origin of the section. The data support the monophyly of sect. Tridentatae, with the exclusion of A. bigelovii and A. palmeri-two historically, anomolous species. However, the ITS data provide insufficient variation to fully resolve interspecific relationships or to support major lineages within the Tridentata clade. Nuclear and chloroplast DNA phylogenies are discordant, which may be a result of interspecific gene flow and subsequent chloroplast capture, particularly related to the placement of A. filifolia and A. californica, in addition to A. bigelovii. Furthermore, the ITS data are in conflict with cpDNA data, providing equivocal evidence for competing hypotheses on the Old World vs. New World origin for the section and do not provide support for definitive subgeneric placement.

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No abstract

Historical and current ecological and genetic factors were examined in western popultions of Eriocaulon kornickianum to determine the cause and consequence of rarity in this disjunct species and to assess its extinction vulnerability. Reproductive biology was examined, which included an examination of breeding systems, seed set, and pollen viability. Recruitment into and maintenance of the populations was evaluated using seed bank estimates. A disturbance study was conducted that examined the effects of competing vegetation and associated species, and included clipping, burning, and artificial seeding. Enzyme electrophoresis was used to measure genetic variation in an effort to examine gene flow within and among populations. Many factors are contributing to the decline of the western populations of this species and include an annual or weak perennial life history, no vegetative reproduction, low seed set, little seed bank contribution to population growth and maintenance, genetic homogeneity, a restricted habitat, and poor competitive abilities. Continuing studies of genetic variation are being used to examine historical factors that may have resulted in a genetic bottleneck observed in the western populations.