The design and fabrication of a membrane-integrated microfluidic cell culture device (five layers, 500 lm total thickness) developed for high resolution microscopy is reported here. The multi-layer device was constructed to enable membrane separated cell culture for tissue mimetic in vitro model applications and pharmacodynamic evaluation studies. The microdevice was developed via a unique combination of low profile fluidic interconnect design, substrate transfer methodology, and wet silane bonding. To demonstrate the unique high resolution imaging capability of this device, we used oil immersion microscopy to image stained nuclei and mitochondria in primary hepatocytes adhered to the incorporated membrane V C 2011 American Institute of Physics.

Oxidation of pyrite-rich, carbonaceous mudrocks used as fill beneath ground bearing floor slabs is currently causing significant problems to domestic and other properties in Ireland. Due to the precipitation of gypsum and other minerals the fill expands and this causes heave of slabs, together with distortion and cracking of the structure. Temperature is known to have an effect on both the rate and amount of this expansion; however, the full impact of human habitation on this process is not fully understood. The paper gives details of the development and testing of a system for monitoring temperature, humidity and pressure conditions within the material. This system is designed to minimise cost and disruption to the homeowner, whilst facilitating reliable measurements over period of 3 to 12 months.

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