The aim of this paper was to evaluate the skin irritant potential of five plant extracts for cosmetic use by in vitro and in vivo methods and to analyze the correlation between these different methods. Cell viability in fibroblasts and in the reconstructed human skin model was measured using the MTT assay to test cytotoxicity, and ELISA was used to detect IL-8 release. Finally, human skin patch tests were performed to validate the results obtained using the in vitro methods. The human patch results were compared with IL-8 release results in fibroblasts and the MTT assay findings in Episkin TM . The MTT assay in fibroblasts showed that the five plant extracts exhibited low cytotoxicity. The human patch results showed that four of the five plant extracts showed weak irritant potential. The MTT assay results in Episkin TM were best correlated with the human patch test results with a Spearman's correlation coefficient of 0.6156. The Spearman's correlation coefficient between the human patch test and IL-8 release results was 0.4617. The MTT assay in Episkin TM was more sensitive than the IL-8 release assay in fibroblasts to evaluate skin irritant potential. Our findings indicate that the five plant extracts are safe for cosmetic use.