Abstract. Stroke is one of the leading causes of mortality worldwide. Protective agents that can diminish injuries caused by cerebral ischemia-reperfusion (I/R) are important in alleviating the harmful outcomes of stroke. The aim of the present study was to investigate the protective role of sanguinarine in cerebral I/R injury. A rat middle cerebral artery occlusion model was used to assess the clinical effect of sanguinarine, and inflammatory cytokines in the serum were detected by ELISA. Western blotting was performed to examine the change in levels of apoptosis-associated proteins in the injured brains. The results suggested that sanguinarine, an anti-inflammatory agent derived from the roots of Sanguinaria canadensis, improved the state of cerebral ischemia in a rat model. The data demonstrated that when rats were treated with sanguinarine prior to middle cerebral artery occlusion, the infarct volume was reduced significantly. The inflammatory factors tumor necrosis factor-α, interleukin (IL)-6 and IL-1β were measured in sanguinarine and vehicle-treated groups using an enzyme-linked immunosorbent assay, and the expression levels of the three factors were significantly reduced following treatment with sanguinarine (P<0.05). In addition, western blot analysis demonstrated that the ratio of B-cell lymphoma 2/Bcl-2-associated X protein was significantly increased following treatment with sanguinarine (P<0.05). The study demonstrated that sanguinarine exerts a protective effect in cerebral ischemia, and that this effect is associated with the anti-inflammatory and anti-apoptotic properties of sanguinarine.

Information on the spatial variability of soil-available micronutrients is important for farming and soil management practices. As current knowledge of factors influencing soil available micro-nutrients in the long-term scales is limited, we analyzed 821 and 812 representative surface (0-20 cm) soil samples from five sub-catchments in the Ping Gu intermontane basin in Beijing, China in 2007 and 2017. The objectives of this study were to assess the temporal and spatial distribution characteristics of soil-available micronutrients (Cu, Zn, Fe and Mn) and their relationships with soil's chemical properties. The concentration of available Cu ranged from 1-2 mg·kg −1 distributing over a large area in 2007, but it was more than 2 mg·kg −1 in the hilly regions in 2017. The concentration of available Zn (>5 mg·kg −1 ) increased significantly from 2007 to 2017, and showed an uneven distribution. The distribution of available Fe and Mn decreased from the northeast to the southwest region of the study area between 2007 and 2017, this being consistent with the topography in this area. Soil's available P (AP) had a higher contribution to available Cu and Zn in different sub-catchments. In addition, soil pH had a significant negative influence on available Fe in sub-catchments 1, 2 and 3, and on available Mn in all sub-catchments, except for sub-catchment 4. Moreover, the effects of soil chemical properties on soil-available micronutrients increased in each sub-catchment from 2007 to 2017. We conclude that differences in soil properties and land-use types were the main reasons for the spatial variability of soil-available micronutrients in the Ping Gu intermontane basin.

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