Group A rotaviruses are the main causative agent of infantile gastroenteritis. The segmented nature of the viral genome allows reassortment of genome segments, which can generate genetic variants. In this study, we characterized the diversity of the VP7, VP4 (VP8*), VP6, NSP4, and NSP5 genes of the rotaviruses that circulated from 2005 to 2011 in the Triângulo Mineiro (TM) region of Brazil. Samples with genotypes G2 (sublineages IVa-1 and IVa-3), G1 (sublineage I-A), G9 (lineage III), G12 (lineages II and III), G8 (lineage II), G3 (lineage III), P[4] (sublineages IVa and IVb), P[8] (sublineages P[8]-3.6, P[8]-3.3, and P[8]-3.1), I2 (lineage VII), E2 (lineages VI, XII, and X), and H2 (lineage III) were identified. The associations found in the samples were G1, G9, or G12 with P[8]-I1-E1-H1; G2 or G8 with P[4]-I2-E2-H2; G12 with I3-E3-H6; and G3 with P[4]-I2-E3-H3 (previously unreported combination). Reassortment events in G2P[4] strains and an apparent pattern of temporal segregation within the lineages were observed. Five TM samples contained genes that exhibited high nucleotide and amino acid identities with strains of animal origin. The present study includes a period of pre- and post-introduction of rotavirus vaccination in all Brazilian territories, thereby serving as a basis for monitoring changes in the genetic constitution of rotaviruses. The results also contribute to the understanding of the diversity and evolution of rotaviruses in a global context.
Rotaviruses are the main cause of infantile acute diarrhea, and a monovalent (G1P [8]) vaccine against the virus was introduced into the Brazilian National Immunization Program for all infants in March 2006. The objectives of this study were to determine the rate and genotype distribution of rotavirus causing infantile diarrhea in the Triaˆngulo Mineiro region of Brazil during 2011-2012 and to assess the impact of local vaccination. Fecal specimens were analyzed for detection and characterization of rotavirus using polyacrylamide gel electrophoresis, reverse transcription followed by polymerase chain reaction (PCR), and PCR-genotyping assays. Overall, rotavirus was diagnosed in 1.7% (6/348) of cases.
Introdução: O dengue é uma doença infecciosa frequente no Brasil e, particularmente, na cidade de Uberaba, Minas Gerais. A doença se manifesta em um amplo espectro de sintomas que levam a alterações nos parâmetros do hemograma. Em nosso trabalho, procuramos estudar a frequência de cada uma das alterações nas séries vermelha, branca e plaquetária em pacientes acometidos pelo dengue em Uberaba-MG, comparando os biênios de 2005-2006 e 2009-2010. Métodos: Foram analisados hemogramas de pacientes atendidos em laboratório particular e em Unidade de Pronto Atendimento da cidade, que apresentaram resultado positivo para sorologia de dengue (IgM). Resultados: Nos biênios 2005-2006 e 2009-2010, foram selecionados, respectivamente, 1.061 e 208 hemogramas para inclusão no estudo. Leucopenia, linfopenia, monocitose e plaquetopenia foram as alterações mais frequentemente encontradas. Conclusão: Algumas alterações apresentaram valor como suporte ao diagnóstico da doença em casos nos quais seria difícil ou impossível a realização de exame sorológico para sua detecção.
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