The evaluation of workers as potential reservoirs and disseminators of pathogenic bacteria has been described as a strategy for the prevention and control of healthcare-associated infections (HAIs). The aim of this study was to evaluate the presence of Enterobacteriaceae in the oral cavity of workers at an oncology hospital in the Midwest region of Brazil, as well as to characterize the phenotypic profile of the isolates. Saliva samples of 294 workers from the hospital’s healthcare and support teams were collected. Microbiological procedures were performed according to standard techniques. Among the participants, 55 (18.7%) were colonized by Enterobacteriaceae in the oral cavity. A total of 64 bacteria were isolated, including potentially pathogenic species. The most prevalent species was Enterobacter gergoviae (17.2%). The highest rates of resistance were observed for β-lactams, and 48.4% of the isolates were considered multiresistant. Regarding the enterobacteria isolated, the production of ESBL and KPC was negative. Nevertheless, among the 43 isolates of the CESP group, 51.2% were considered AmpC β-lactamase producers by induction, and 48.8% were hyper-producing mutants. The significant prevalence of carriers of Enterobacteriaceae and the phenotypic profile of the isolates represents a concern, especially due to the multiresistance and production of AmpC β-lactamases.

Sugarcane (Saccharum sp, Poaceae) is native to Southeast Asia, and due to growing demand as raw material, its cultivation recently expanded to new frontiers. The genetic diversity analysis is essential for targeting strategies in the formation and maintenance of a germplasm. This study aimed to assess the genetic diversity of 26 accessions of sugarcane from the Active Germplasm Bank of Embrapa Coastal Tablelands, using inter-simple sequence repeat (ISSR) molecular markers. Sixteen primers were used, resulting in 87 fragments with 91.13% of polymorphism. The similarity of the individuals ranged between 0.22 and 0.87. Individuals RB867515 and RB92579 were closer genetically, and the most distant ones were PI240785 and NSL 291970. Four distinct clusters were formed, using UPGMA. This information can be used to prioritize the selection of accessions for the conduction of hybridization in breeding and germplasm exchange actions.

RESUMO ABSTRACT Objectives:To assess colonization by Staphylococcus aureus sensitive and resistant to methicillin found in the saliva of nurses and, to recognize the mupirocin profile of resistance. Methods: It was collected three saliva samples of 356 (94.2%) professional research participants, totaling 1068 samples processed. All ethical aspects were covered. Microbiological analysis included the phenotypic identification, according to the standards of the Clinical and Laboratory Standards Institute. Results: In the first collection were obtained 73 individuals colonized by Staphylococcus aureus, 48 in the second and 37 in the third, totaling 158 samples of Staphylococcus aureus. Of these, 24 (15.2%) were resistant to oxacillin and 30 resistant to mupirocin (18.9%). Mupirocin resistance was observed sharply in the Staphylococcus aureus resistant to methicillin (MRSA); of 24 MRSA, 17 (70.8%) were resistant to this antibiotic. Conclusion: The results indicated that mupirocin resistance is a major problem since this antibiotic is an excellent ally in the measures taken to control infection in health services. Keywords: Staphylococcus aureus; Drug resistance, multiple; Mupirocin ; Nursing team RESUMEN Objetivos: Evaluar la colonización por Staphylococcus aureus sensibles y resistentes al meticilín en la saliva de profesionales de enfermería y, reconocer el perfil de resistencia a la mupirocina. Métodos: Fueron recolectadas tres muestras de saliva de 356 (94,2%) profesionales participantes de la investigación, totalizando 1.068 muestras procesadas. Todos los aspectos éticos fueron contemplados. El análisis microbiológico comprendió la identificación fenotípica, según normas del Clinical and Laboratory Standards Institute. Resultados: Fueron obtenidos 73 individuos colonizados por Staphylococcus aureus en la primera recolección, 48 en la segunda y 37 en la tercera, sumando 158 muestras aisladas de Staphylococcus aureus. De esas, 24 (15,2%) eran resistentes a la oxacilina y 30 resistentes a al mupirocina (18,9%). La resistencia al mupirocina fue observada de forma pronunciada en los Staphylococcus aureus resistentes a la metacilina (MRSA); de los 24 MRSA, 17 (70,8%) se presentaron resistentes a este antimicrobiano. Conclusión: Los resultados indicaron que la resistencia a la mupirocina es un importante problema, ya que ese antibiótico es un excelente aliado en las medidas de control de las infecciones de servicios de salud.

The use of plant essential oils has been adopted as less hazardous to the environment and human health than synthetic insecticides used for the control of insects that transmit diseases. Despite of exerting insecticidal activities against several insect disease vectors, the potential impacts on non-target organisms exerted by essential oils extracted from Lippia sidoides (Cham.) have not received adequate attention. Here, we evaluated the susceptibility and potential changes in consumption rates of honey bees, Apis mellifera (L.), when exposed to essential oils extracted from L. sidoides. Was exposed forager bees to honey syrup (50% v/v) containing L. sidoides essential oil for 5 h. After this exposure period, the bees received regular honey syrup for another 19 h period. Six essential oil concentrations was used, namely 1.0, 1.5, 2.0, 2.5, 3.0 and 3.5 µL of essential oil/mL of syrup, and evaluated the syrup consumption and bees mortality in both periods (at the 5th and 24th h). The results reveal that independent of the essential oil concentration, the forager bees fed significantly less on L. sidoides essential oil-containing honey syrup. However, feeding on L. sidoides essential oil-containing honey syrup did not cause significant mortality when compared with bees that were not exposed to the essential oils. Thus, the results demonstrate that L. sidoides essential oils exhibited adequate selectivity against honey bees.

O objetivo deste estudo foi caracterizar o perfil dos trabalhadores de um hospital oncológico colonizados na cavidade bucal por Enterobacteriaceae. Foram investigadas as variáveis colonização bacteriana, sóciodemográficas, profissional, de doença/infecção e comportamental. A coleta de saliva e as análises microbiológicas foram realizadas por técnicas padronizadas; a coleta de dados, por meio da aplicação de formulário. Dentre os 55 trabalhadores colonizados por Enterobacteriaceae, 56,4% (31/55) também albergavam na cavidade bucal Staphylococcus e/ou Pseudomonas. A categoria profissional mais comumente colonizada foi a de técnico de enfermagem. Quadros frequentes de doença/infecção foram relatados entre os portadores. Os dados apresentados revelaram uma realidade preocupante para o contexto da assistência à saúde. Considera-se que estes resultados contribuem com subsídios importantes para os programas de prevenção e controle de infecção, visto que o conhecimento do estado de portador reduz os riscos de transmissão de micro-organismos.