Shiga toxin-producing Escherichia coli (STEC) D157:H7 strains (isolated by cattle's faeces and a reference strain, EDL933), were inoculated into pasteurized milk (10 2 and 10 3 cells.mL -1) to prepare the Minas frescal cheese. As control was used uninfected milk. Physicochemical and microbiological analyses were performed to milk and elaborated cheese. The D157:H7 strains were quantified in the stages of cheese processing and during 0, 2, 4, 5, 7, 10 and 15 storage days at 8 °C onto Sorbitol MacConkey Agar supplemented with potassium tellurite and cefixime (CT-SMAC). D157:H7 was not present in the pasteurised milk prior to the artificial inoculation. At the end of the processing the cheese had 10 to 100 times more STEC D157:H7 than the initial inoculum. During the storage, the Minas frescal cheese exhibited the largest population increase on the 4th and 5th day when inoculated with 10 2 and 10 3 cells.mL -1, respectively. Additionally, viable cells were found up to the 10th and 15th day, according to the amount of initial inoculum. This number of cells is able to cause infection in humans, and therefore, Minas frescal cheese, even when stored under refrigeration, is a potential vehicle of disease caused by STEC D157:H7.Keywords: cheese; psychrotrophic bacteria; foodborne disease. Practical Application:This study demonstrates that the Minas frescal cheese may be an important vehicle for STEC D157:H7, since this microorganism remains viable in this food for a long period even under refrigeration. In this study we can observe the psychrotrophic behavior of STEC D157:H7 in this rich food which is Minas frescal cheese.
Objetivo: Descrever o perfil antropométrico e a composição corporal de idosos atendidos no Ambulatório de Nutrição do Centro de Referência em Assistência à Saúde do Idoso da Universidade Federal Fluminense. Metodologia: Trata-se de um estudo transversal, realizado com 52 indivíduos com 60 anos ou mais atendidos no ambulatório de nutrição. As medidas antropométricas foram realizadas segundo as diretrizes da International Society for the Advancement of Kineanthropometry, considerando as seguintes variáveis: massa corporal, estatura, dobras cutâneas biciptal, triciptal, subescapular, supra-ilíaca, percentual de gordura corporal a partir do somatório das quatro dobras cutâneas, circunferência da cintura e circunferência do braço. As análises estatísticas foram realizadas utilizando o software Graph Pad inStat ® , versão 3.1, considerando-se significativo p < 0,05. Resultados: Verificou-se prevalência de sobrepeso (43,5% da amostra), adequação da circunferência do braço, circunferência muscular do braço e dobra cutânea triciptal. A maior parte das mulheres (67,6%) apresentava risco elevado para doenças cardiovasculares segundo a circunferência abdominal. Observou-se percentual de gordura corporal elevado em ambos os sexos (89,2% e 66,7%, feminino e masculino, respectivamente). Conclusão: Observou-se a presença de inadequações do estado nutricional da população em estudo de acordo com os parâmetros antropométricos índice de massa corporal, circunferência abdominal e percentual de gordura pelo somatório de dobras.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.