The presence of Listeria spp. in chicken drumsticks, wings, breasts, and livers taken from a poultry processing plant was investigated. The poultry pieces found to be most contaminated were drumstick meat and skin, with 96% of samples shown to contain Listeria spp. Drumsticks contained the highest number of Listeria spp., with counts in many cases exceeding 3 log CFU/g for skin and muscle. Samples were also taken from various surfaces in the processing plant, where the most contaminated areas were found to be in the final stages of meat processing. Three samples were positive for Listeria spp. immediately after washing and disinfection had taken place. The results suggest that drumsticks are responsible for a large amount of the contamination of chicken carcasses, and that the surfaces that come into contact with these pieces of meat play an important role in spreading Listeria spp. Disinfection procedures used by this processing plant do not effectively control Listeria spp.
In recent years, the use of nitrites and sulfites as food preservatives has been a cause for concern due to the health problems that these additives can cause in humans. Natural products have been studied as an alternative, but most of them have hardly been applied in the food industry for technological and economic reasons. In this sense, organic salts such as sodium acetate are a good alternative due to their affordability. Thus, this study evaluated the capacity of sodium nitrite, sodium sulfite, a sodium acetate product (TQI C-6000), and chitosan to inhibit two important foodborne pathogens, Salmonella enterica and Listeria monocytogenes. The MIC of each chemical was in vitro evaluated and their antibacterial action was subsequently checked in situ using minced meat as a food model. MIC values of sodium nitrite (10,000 mg/L) and sodium sulfite (50,000 mg/L) for Salmonella enterica were higher than the values allowed by legislation (450 mg/L for sulfites and 150 mg/L for nitrites). Additionally, the sodium acetate product caused the inhibition of Salmonella enterica and Listeria at a relative low quantity. The two foodborne pathogens were inhibited in the food model with 1% of the sodium acetate product. Additionally, there were no significant differences between sodium nitrite, sodium sulfite, and sodium acetate products in the inhibition of Salmonella enterica and Listeria monocytogenes in the food model. Thus, products based on sodium acetate can be an alternative to traditional preservatives in food products.
Salmonella spp. is a major food-borne pathogen around the world. The ability of Salmonella to produce biofilm is one of the main obstacles in reducing the prevalence of these bacteria in the food chain. Most of Salmonella biofilm studies found in the literature used laboratory growth media. However, in the food chain, food residues are the principal source of nutrients of Salmonella. In this study, the biofilm formation, morphotype, and motility of 13 Salmonella strains belonging to three different subspecies and isolated from poultry houses was evaluated. To simulate food chain conditions, four different growth media (Tryptic Soy Broth at 1/20 dilution, milk at 1/20 dilution, tomato juice, and chicken meat juice), two different surfaces (stainless steel and polystyrene) and two temperatures (6 °C and 22 °C) were used to evaluate the biofilm formation. The morphotype, motility, and biofilm formation of Salmonella was temperature-dependent. Biofilm formation was significantly higher with 1/20 Tryptic Soy Broth in all the surfaces and temperatures tested, in comparison with the other growth media. The laboratory growth medium 1/20 Tryptic Soy Broth enhanced biofilm formation in Salmonella. This could explain the great differences in biofilm formation found between this growth medium and food residues. However, Salmonella strains were able to produce biofilm on the presence of food residues in all the conditions tested. Therefore, the Salmonella strain can use food residues to produce biofilm on common surfaces of the food chain. More studies combining more strains and food residues are necessary to fully understand the mechanism used by Salmonella to produce biofilm on the presence of these sources of nutrients.
Esta investigación analiza la información política e institucional en los telediarios de TVE para conocer el tratamiento de los acontecimientos que protagonizan los partidos políticos, las instituciones y sus representantes. Metodología. Trabajamos con una metodología mixta. El análisis cuantitativo es necesario para determinar el grado de pluralidad formal y el cualitativo sirve para conocer los detalles de los enfoques de las noticias. Además, incluimos un grupo de discusión formado por cinco redactores para averiguar cómo funciona el proceso informativo en la televisión pública. Resultados y conclusiones. Los datos demuestran que el 97% de las noticias tienen un tratamiento formal correcto, pero estas cifras contrastan con los resultados del análisis de contenido. El 74% de los temas de información institucional tienen un enfoque que favorece, refuerza o avala la imagen del Gobierno y de los organismos afines.
Pages 1057 consumption habits, and whether this influence is reflected on students' routines, interest and preferences. Results and conclusions. The press, radio and television continue losing audiences and most students now get the news from digital media platforms as they are already disconnected from traditional news media outlets. Technological developments have enabled new ways of dealing with space and managing time, which have an impact on the way people access information, on news content itself and on people's possibilities to learn about what happens around them.
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