The objective of the present work was to offer an in vitro propagation alternative for the Oncidium sphacelatum orchid through the evaluation of the response to the crop in different concentrations of sucrose and the nutrient medium of Murashige and Skoog. Initially the seeds were sown in this culture medium at 100% of their concentration with the addition of 3.0% sucrose, once formed the protocorms the response to the application of different sucrose concentrations was evaluated (3.0, 2.25, 1.5, 0.75 and 0%) and of the nutrients of the culture medium (100, 75, 50, 25 and 0%) under a completely randomized experimental design of eight treatments with four repetitions. The analysis of variance showed statistical differences between treatments for the formation of buds in the protocorms, number of leaves per bud, plant height, root length and formation of pseudobulbs in the shoots. The highest outbreak formation in the protoorms was observed in treatments with 3.0% sucrose and nutrient concentrations at 50 or 25%. For the growth of the leaves and the root the concentration of 3.0% of sucrose was required combined with the concentrations of nutrients at 25, 50 and 100%. The formation of pseudobulbs in the outbreaks only occurred in the treatment with 3.0% sucrose combined with the 25% nutrient concentration.