Characterization of a new tomato () T-DNA mutant allowed for the isolation of the () gene whose lack of function was responsible for the severe alterations observed in the shoot apex and reproductive organs under salinity conditions. Physiological studies proved that gene is required to maintain a proper low Na/Ca ratio in growing tissues allowing tomato growth under salt stress. Expression analysis of the main responsible genes for Na compartmentalization (i.e. ,, , [] and V-ATPase []) supported a reduced capacity to accumulate Na in mutant leaves, which resulted in a lower uploading of Na from xylem, allowing the toxic ion to reach apex and flowers. Likewise, the tomato and (), key genes for Ca fluxes to the vacuole, showed abnormal expression in plants indicating an impaired Ca release from vacuole. Additionally, complementation assay revealed that is a true ortholog of the Arabidopsis () gene, supporting that the essential function of CBL10 is conserved in Arabidopsis and tomato. Together, the findings obtained in this study provide new insights into the function of in salt stress tolerance. Thus, it is proposed that SlCBL10 mediates salt tolerance by regulating Na and Ca fluxes in the vacuole, cooperating with the vacuolar cation channel and the two vacuolar H-pumps, and, which in turn are revealed as potential targets of .

A dramatic evolution of fruit size has accompanied the domestication and improvement of fruit-bearing crop species. In tomato (Solanum lycopersicum), naturally occurring cis-regulatory mutations in the genes of the CLAVATA-WUSCHEL signaling pathway have led to a significant increase in fruit size generating enlarged meristems that lead to flowers with extra organs and bigger fruits. In this work, by combining mapping-by-sequencing and CRISPR/Cas9 genome editing methods, we isolatedEXCESSIVE NUMBER OF FLORAL ORGANS(ENO), an AP2/ERF transcription factor which regulates floral meristem activity. Thus, theENOgene mutation gives rise to plants that yield larger multilocular fruits due to an increased size of the floral meristem. Genetic analyses indicate thatenoexhibits synergistic effects with mutations at theLOCULE NUMBER(encodingSlWUS) andFASCIATED(encodingSlCLV3) loci, two central players in the evolution of fruit size in the domestication of cultivated tomatoes. Our findings reveal that anenomutation causes a substantial expansion ofSlWUSexpression domains in a flower-specific manner. In vitro binding results show that ENO is able to interact with the GGC-box cis-regulatory element within theSlWUSpromoter region, suggesting that ENO directly regulatesSlWUSexpression domains to maintain floral stem-cell homeostasis. Furthermore, the study of natural allelic variation of theENOlocus proved that a cis-regulatory mutation in the promoter ofENOhad been targeted by positive selection during the domestication process, setting up the background for significant increases in fruit locule number and fruit size in modern tomatoes.

SummaryWith the completion of genome sequencing projects, the next challenge is to close the gap between gene annotation and gene functional assignment. Genomic tools to identify gene functions are based on the analysis of phenotypic variations between a wild type and its mutant; hence, mutant collections are a valuable resource. In this sense, T‐DNA collections allow for an easy and straightforward identification of the tagged gene, serving as the basis of both forward and reverse genetic strategies. This study reports on the phenotypic and molecular characterization of an enhancer trap T‐DNA collection in tomato (Solanum lycopersicum L.), which has been produced by Agrobacterium‐mediated transformation using a binary vector bearing a minimal promoter fused to the uidA reporter gene. Two genes have been isolated from different T‐DNA mutants, one of these genes codes for a UTP‐glucose‐1‐phosphate uridylyltransferase involved in programmed cell death and leaf development, which means a novel gene function reported in tomato. Together, our results support that enhancer trapping is a powerful tool to identify novel genes and regulatory elements in tomato and that this T‐DNA mutant collection represents a highly valuable resource for functional analyses in this fleshy‐fruited model species.

Ca2+ is a second messenger that mediates plant responses to abiotic stress; Ca2+ signals need to be decoded by Ca2+ sensors that translate the signal into physiological, metabolic, and molecular responses. Recent research regarding the Ca2+ sensor CALCINEURIN B-LIKE PROTEIN 10 (CBL10) has resulted in important advances in understanding the function of this signaling component during abiotic stress tolerance. Under saline conditions, CBL10 function was initially understood to be linked to regulation of Na+ homeostasis, protecting plant shoots from salt stress. During this process, CBL10 interacts with the CBL-interacting protein kinase 24 (CIPK24, SOS2), this interaction being localized at both the plasma and vacuolar (tonoplast) membranes. Interestingly, recent studies have exposed that CBL10 is a regulator not only of Na+ homeostasis but also of Ca2+ under salt stress, regulating Ca2+ fluxes in vacuoles, and also at the plasma membrane. This review summarizes new research regarding functions of CBL10 in plant stress tolerance, predominantly salt stress, as this is the most commonly studied abiotic stress associated with the function of this regulator. Special focus has been placed on some aspects that are still unclear. We also pay particular attention on the proven versatility of CBL10 to activate (in a CIPK-dependent manner) or repress (by direct interaction) downstream targets, in different subcellular locations. These in turn appear to be the link through which CBL10 could be a key master regulator of stress signaling in plants and also a crucial participant in fruit development and quality, as disruption of CBL10 results in inadequate Ca2+ partitioning in plants and fruit. New emerging roles associated with other abiotic stresses in addition to salt stress, such as drought, flooding, and K+ deficiency, are also addressed in this review. Finally, we provide an outline of recent advances in identification of potential targets of CBL10, as CBL10/CIPKs complexes and as CBL10 direct interactions. The aim is to showcase new research regarding this master regulator of abiotic stress tolerance that may be essential to the maintenance of crop productivity under abiotic stress. This is particularly pertinent when considering the scenario of a projected increase in extreme environmental conditions due to climate change.

Summary CRABS CLAW (CRC) orthologues play a crucial role in floral meristem (FM) determinacy and gynoecium formation across angiosperms, the key developmental processes for ensuring successful plant reproduction and crop production. However, the mechanisms behind CRC mediated FM termination are far from fully understood. Here, we addressed the functional characterization of tomato (Solanum lycopersicum) paralogous CRC genes. Using mapping‐by‐sequencing, RNA interference and CRISPR/Cas9 techniques, expression analyses, protein–protein interaction assays and Arabidopsis complementation experiments, we examined their potential roles in FM determinacy and carpel formation. We revealed that the incomplete penetrance and variable expressivity of the indeterminate carpel‐inside‐carpel phenotype observed in fruit iterative growth (fig) mutant plants are due to the lack of function of the S. lycopersicum CRC homologue SlCRCa. Furthermore, a detailed functional analysis of tomato CRC paralogues, SlCRCa and SlCRCb, allowed us to propose that they operate as positive regulators of FM determinacy by acting in a compensatory and partially redundant manner to safeguard the proper formation of flowers and fruits. Our results uncover for the first time the physical interaction of putative CRC orthologues with members of the chromatin remodelling complex that epigenetically represses WUSCHEL expression through histone deacetylation to ensure the proper termination of floral stem cell activity.