Existem, aproximadamente, 21.000 plantas medicinais, sendo 800 delas usadas para fins antidiabéticos. O presente estudo tem como objetivo apresentar uma atualização da efetividade da fitoterapia no tratamento do diabetes mellitus utilizando como referência a planta pata de vaca (Bauhinia forficata). Foi realizada uma revisão integrativa, através da busca de artigos nas bases de dados LILACS, MEDLINE e Google Acadêmico, utilizando os descritores “diabetes mellitus” AND “Bauhinia forficata” OR “pata de vaca”. Os artigos apontam redução dos níveis de creatinina, índice HOMA, LDL e colesterol total com o uso da planta. Desse modo, a efetividade na melhora dos sintomas da doença é comprovada, devido às propriedades farmacológicas apresentadas pela pata de vaca. Portanto, faz-se necessário que haja melhorias na implementação de políticas públicas e capacitação profissional para efetiva utilização da fitoterapia no tratamento de doenças crônicas.

Sepsis is a systemic inflammatory disease which affects about 17 million people per year worldwide, with a mortality rate above 50%. Sepsis by gram‐positive bacteria has a higher mortality rate when compared with sepsis by gram‐negative bacteria. However, experimental models that adequately mimic the signs of sepsis by gram‐positive bacteria are limited. An inefficient neutrophils (NE) mobilization in Staphylococcus aureus‐induced sepsis is the main cause of death in patients infected with this bacterium. Staphylococcus aureus pathological effects are strong related to the secretion of staphylococcal enterotoxins (SEs). Recently, we have shown that human eosinophils and mice bone marrow granulocytes incubated with SEs exhibit reduced in vitro chemotaxis and adhesion activity. The present study aims to identify the effects of SEA and SEB on the functional properties of human NE. Blood was collected from healthy volunteers after approval from the local ethics committee (Protocol No 61370616.3.0000.5412). Blood samples were placed on isotonic Percoll solution. After centrifugation, the mononuclear cell layer was removed and the pellet containing erythrocytes and granulocytes was aspirated and subjected to isotonic lysis. After lysis cells (98% NE) were resuspended to 4 × 106 cells/ml and submitted to in vitro incubation with SEA or SEB (100 ng/ml; 2 h). Adhesion assays were carried out in 96‐well plates pre‐coated with recombinant human VCAM‐1 and ICAM‐1 for 30 min in the presence of interleukin‐8 (IL‐8). The NE adhesion was calculated by measuring myeloperoxidase (MPO) activity on adherent cells. NE migration assay was performed using a 48‐well microchemotaxis Boyden chamber. Both SEA and SEB reduces human neutrophil adhesion in VCAM‐1 (Control: 4.7 ± 0.5; untreated NE + IL‐8: 6.7 ± 0.6; SEA treated NE+IL‐8: 4.9 ± 0.8; SEB treated NE + IL‐8: 3.7 ± 0.6*# optic density/NE × 106 cells; ) or ICAM‐1(Control: 4.3 ± 0.5; untreated NE + IL‐8: 6.1 ± 0.6; SEA treated NE+IL‐8: 5.5 ± 1.0; SEB treated NE + IL‐8: 3.7 ± 0.9 optic density/NE × 106 cells) coated plates. Similar results were observed for NE chemotactic activity (Control: 18.7 ± 0.8; untreated NE +IL‐8: 52.9 ± 2.7; SEA treated NE+IL‐8: 28.5 ± 1.4; SEB treated NE + IL‐8: 34.2 ± 1.4 NE per high‐power field). The inhibitory effect of SEA and SEB on human NE in vitro adhesion and chemotaxis suggests a role of these toxins on the neutrophil dysfunction associated with the severe sepsis by Staphylococcus aureus.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Interferon γ (IFN‐g) is a cytokine produced by natural killer T cells implicated in innate and acquired immune response during bacterial infections. Higher levels of this cytokine are suggested to suppress the hematopoiesis, leading to leukopenia that is clinically observed after the resolution of severe infectious. Evidence shows that IFN‐g induces MHC class II overexpression in human circulating neutrophils (NE) and reduces Staphylococcus aureus‐mouse induced NE infiltration. Our preliminary results have been showing that staphylococcal enterotoxins (SEs; type A and B) are able to inhibit human NE properties such as adhesion and chemotactic response. Whereas, IFN‐g release is reported to contribute to pathological events induced by SEs the aim of the present study was investigate the effect of IFN‐g on the functional properties of human NE. Blood was collected from healthy volunteers after approval from the local ethics committee (Protocol No 61370616.3.0000.5412). Blood samples were placed on isotonic Percoll solution. After centrifugation, the mononuclear cell layer was removed and the pellet containing erythrocytes and granulocytes was aspirated and subjected to isotonic lysis. After lysis cells (98% NE) were resuspended to 4 × 106 cells/ml and submitted to in vitro incubation with IFN‐g (10000 IU/ml; 30 min). Adhesion assays were carried out in 96‐well plates pre‐coated with recombinant human VCAM‐1 and ICAM‐1 for 30 min in the presence of interleukin‐8 (IL‐8). The NE adhesion was calculated by measuring myeloperoxidase (MPO) activity on adherent cells. NE migration assay was performed using a 48‐well microchemotaxis Boyden chamber. Prior NE in vitro incubation with IFN‐g reduces human NE adhesion in VCAM‐1 (Control: 4.7 ± 0.5; untreated NE + IL‐8: 6.7 ± 0.6; IFN‐g treated NE+IL‐8: 4.8 ± 0.66 optic density/NE × 106 cells; ) or ICAM‐1(Control: 4.3 ± 0.5; untreated NE + IL‐8: 6.1 ± 0.6; IFN‐g treated NE+IL‐8: 3.9 ± 0.48 optic density/NE × 106 cells) coated plates. Similar results were observed for NE chemotactic activity (Control: 18.7 ± 0.8; untreated NE +IL‐8: 52.9 ± 2.7; IFN‐g treated NE+IL‐8: 36.4 ± 1.6 NE per high‐power field). These findings support the hypothesis that IFN‐g modulates the inhibitory effects of Staphylococcal enterotoxins on NE functional properties.Support or Funding InformationCAPES‐Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (1676187).This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

A Faculdade de Medina de Jundiaí realizará no dia 18 de setembro de 2020, sextafeira, o XVI Fórum de Iniciação Científica PIBIC-FMJ-CNPq. Devido à pandemia do novo coronavírus e à necessidade de distanciamento social, este ano o evento será realizado on-line, através de videoconferências no Google Meet. Voltado aos alunos e professores dos cursos de Graduação e Pós-Graduação da FMJ, o evento tem como objetivo reunir a comunidade acadêmica da Faculdade para a apresentação dos trabalhos de Iniciação Científica, pertinentes à vigência 2019/2020. A organização do evento é de responsabilidade do Comitê Institucional de Pesquisa Científica -CIP da FMJ.

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