Neves, Notch and Hedgehog in the thymus/parathyroid common primordium: Crosstalk in organ f o r m a t i o n , Developmental Biology, http://dx.doi.org/10. 1016/j.ydbio.2016.08.012 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractThe avian thymus and parathyroids (T/PT) common primordium derives from the endoderm of the third and fourth pharyngeal pouches (3/4PP). The molecular mechanisms that govern T/PT development are not fully understood. Here we study the effects of Notch and Hedgehog (Hh) signalling modulation during common primordium development using in vitro, in vivo and in ovo approaches. The impairment of Notch activity reduced Foxn1/thymus-fated and Gcm2/Pth/parathyroid-fated domains in the 3/4PP and further compromised the development of the parathyroid glands. When Hh signalling was abolished, we observed a reduction in the Gata3/Gcm2-and Lfng-expression domains at the median/anterior and median/posterior territories of the pouches, respectively. In contrast, the Foxn1 expression- This study offers novel evidence on the role of Notch signalling in T/PT common primordium development, in an Hh-dependent manner.

Cystathionine beta-synthase (CBS) catalyzes the formation of cystathionine from homocysteine and serine. CBS is allosterically activated by S-adenosylmethionine (SAM), which binds to its C-terminal regulatory domain. Mutations in this domain lead to variants with high residual activity but lacking SAM activation. We characterized six C-terminal CBS variants (p.P427L, p.D444N, p.V449G, p.S500L, p.K523Sfs*18, and p.L540Q). To understand the effect of C-terminal mutations on the functional/structural properties of CBS, we performed dynamic light scattering, differential scanning fluorimetry, limited proteolysis, enzymatic characterization, and determination of SAM-binding affinity. Kinetic data confirm that the enzymatic function of these variants is not impaired. Although lacking SAM activation, the p.P427L and p.S500L were able to bind SAM at a lower extent than the wild type (WT), confirming that SAM binding and activation can be two independent events. At the structural level, the C-terminal variants presented various effects, either showing catalytic core instability and increased susceptibility toward aggregation or presenting with similar or higher stability than the WT. Our study highlights as the common feature to the C-terminal variants an impaired binding of SAM and no increase in enzymatic activity with physiological concentrations of the activator, suggesting the loss of regulation by SAM as a potential pathogenic mechanism.

Resumo O objectivo deste estudo foi recolher os atributos culturalmente associados ao estereotipo de IntroduçãoOs estereótipos têm sido um dos principais objectos de estudo na literatura da Psicologia Social (e.g., McGarty, Yzerbyt, & Spears, 2002) e, particularmente, da Cognição Social (e.g., Hamilton, 1981 Laboratório de Psicologia, 6(1): 3-13 (2008) © 2008 Traços e estereotípicos a associados a a 3 32 g grupos p profissionaisSérgio Moreira Leonel Garcia-Marques Instituto Superior de Ciências do Trabalho e da Empresa, LisboaAna Sofia Santos Faculdade de Psicologia e Ciências da Educação da Universidade de LisboaA correspondência relativa a este artigo deverá ser enviada para: Sérgio Moreira,

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A sobrequalificação percebida é alvo de debate ao longo dos anos. Apesar de relevante no contexto das migrações, devido aos riscos da experiência migratória, os estudos que a focam são escassos. O estudo 1 valida para o contexto português o Questionário de Sobrequalificação Cognitiva Percebida (QSCP) através de uma amostra de conveniência (N=142). O estudo 2 com abordagem qualitativa explora as relações entre sobrequalificação percebida, satisfação no trabalho e justiça organizacional entre imigrantes e não-migrantes, através de entrevistas semiestruturadas (amostra de conveniência, N=27). Os resultados sugerem uma estrutura unifatorial adequada do QSCP, com boa consistência interna e validade convergente e discriminante. No estudo 2, os imigrantes apresentam mais satisfação profissional quando existe sobrequalificação percebida voluntária, se identificam com o país de acolhimento e residem nele há mais de 10 anos. Estudos futuros devem explorar o modelo teórico proposto para o contexto das migrações, e respetivos moderadores.