Stellacyanin from Rhus vernificera is a blue copper protein in which the metal is coordinated to a Cys, two His, and a Gln residue. It displays a low redox potential, a fast electron exchange rate, and a reversible alkaline transition. We have studied this transition in Cu(II)- and Co(II)-stellacyanin by means of electronic and NMR spectroscopy. The data indicate that a conformational rearrangement of the metal site occurs at high pH. A drastic alteration in the Gln coordination mode, as initially proposed, is discarded. These results show that the metal site in stellacyanin is more flexible than the sites of other blue copper proteins. The present study demonstrates that the paramagnetic shifts of the bound Cys in the Co(II) derivative are sensitive indicators of the electron delocalization and conformational changes experienced by this residue.

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Lotus glaber is a glycophytic, perennial legume from Europe that occurs widely in saline habitats. We evaluated the effect of mycorrhizal fungus colonization on the response to salt stress of two genotypes of L. glaber differing in their tolerance to salinity. The experiment consisted of a randomized block design with two factors: (1) mycorrhizal fungus treatments (with or without AM fungus) and (2) two salinity levels of 0 and 200 mM NaCl. Our results indicated that Glomus intraradices established a more efficient symbiosis with the tolerant than with the sensitive genotype. G. intraradices improved growth of L. glaber plants under saline conditions. They showed higher values of net growth, shoot/root and K + / Na + ratios, and protein concentrations than controls. Tolerant AM plants also showed higher chlorophyll levels than non-AM ones. Prevention of Na + accumulation in the plant and enhancement of K + concentrations in roots observed in this work could be part of the general mechanism of salt stress alleviation of L. glaber by G. intraradices.