The methods applied for DNA extraction from must and wine samples with monovarietal origin are presented and discussed aiming to prove the quality of extracted DNA and its good properties for further use in molecular tests. In the present research were compared four different DNA extraction methods from must and wine samples obtained from eleven V. vinifera varieties (five grapevine varieties for white wines and six grapevine varieties for red wines, respectively). Taking into consideration the amounts of obtained DNA, the concentrations and purities of the final DNA extracts, were stood out two modified methods. For all must samples, very efficient was the second method, which allowed obtaining a mean value of 87.9 ng µl-1 for the DNA concentration with 1.55 purity. Among the tested procedures, for monovarietal wine samples, the fourth method proved to be the most efficient which brought a mean value of 64.7 ng μl-1 for DNA concentration with 1.66 purity. This method adequate for wine samples involves two CTAB solution treatments and the RNase treatment applied before DNA resuspension. The DNA from must and wine extracts and the DNA from leaves of the corresponding grapevine varieties were amplified with five specific microsatellite primers (VVS2, VVMD27, VVMD32, VrZAG79 and VrZAG62). The aspects of pattern profiles were analysed in parallel and proved that the extracted DNA was suitable for amplification with these specific V. vinifera primers. The two selected extraction procedures are considered good for research purposes and ensure obtaining of good-quality extracted DNA from musts and one-year old wines.

This study presents the main morphological features and the first molecular investigations of four new tomato varieties (Solanum lycopersicum), aiming to obtain their complete characterisation. Evaluation with the standard descriptors for tomato revealed specific and distinct traits for each analysed variety. The molecular analyses for variety identification started with testing three methods for DNA extraction. With an optimized method, which doesn’t need liquid nitrogen for plant tissue disruption, good quality DNA was obtained, in adequate quantities, and well preserved when stored at -20 °C. To highlight the genetic differences among the analysed tomato varieties, nine RAPD primers and ten SSR primers were tested. Of these, the optimal amplification protocols for five RAPD primers and five SSR primers were established. The amplified products obtained with RAPD primers revealed an average number of bands per primer of 8.8 and a total rate of polymorphism of 59.1%; with OPB10 primer was seen the highest number of DNA bands (11), and with OPA07 primer was registered the highest degree of genetic variability among the studied varieties (77.7%). Two SSR markers (SSR 20 and SSR T107) amplified monomorphic banding patterns corresponding to 170 base pairs and 250 base pairs, respectively, for all varieties; with SSR T7, SSR T62, and SSR T70 primers were generated multiple amplification bands, with a different distribution of the bands into the agarose gel for each analysed tomato variety.

Aiming to evaluate the in vitro regeneration potential, five varieties of tomatoes (Solanum lycopersicum) were studied for their response in anther culture. Anther explants at an early stage of microspore development were inoculated onto three culture media. The first differentiation processes were recorded during the first three weeks of culture, in darkness. The statistical analysis of the data recorded during the anther culture showed significant differences between genotypes regarding their specific response to culture conditions and the significant influence of the initiation medium composition in triggering the differentiation processes. Under the tested conditions were induced: the embryogenic potential in three genotypes (ʻȘtefănești 22ʼ, ʻCostate 21ʼ and ʻChihlimbarʼ) and the indirect organogenesis in two genotypes (ʻArgeș 20ʼ and ʻCostate 21ʼ). Morphological characteristics of anther-derived plants from ʻArgeș 20ʼ variety, grown in greenhouse conditions (growth rate, features of leaf,  flower, and fruit), as well as analyses with nine SSR markers (banding patterns, the coefficient of genetic similarity, and the polymorphism information content) in DNA samples from each regenerant and the donor variety, provided clear evidence of the occurrence of spontaneous genetic variation during in vitro anther culture, and of the existence of somaclonal variation in regenerated plants. The amplified products obtained with SSR primers revealed a total number of scorable bands of 160 and a mean percentage of polymorphic bands of 21.09%. Two out of the nine tested primers, SSR63 and SLM6-7 proved to be efficient in detecting genetic differences not only among regenerants but also between them and the donor genotype.