The present work was carried out to study the sero-prevalence of Haemonchus contortus infection in sheep by Indirect-Enzyme Linked Immuno Sorbent Assay (Indirect-ELISA) using somatic antigen. Out of 100 abomasums screened, 57 found positive for H. contortus adult worms. A total of 250 serum samples which includes, 100 serum samples from local abattoir in and around shimoga region from the animals from which the abomasums were collected and 150 serum samples from migratory sheep were used to detect the circulating antibody against H. contortus by Indirect-ELISA using somatic antigen. Of the 57 sheep harboring adult worms in their abomasums, the serum samples showed positive reaction by Indirect-ELISA with somatic antigen. However, among 43 sheep which are not showing any adult worms of H. contortus in their abomasums, but their 21 serum samples showed positive reaction by Indirect-ELISA. The sensitivity and specificity of Indirect-ELISA was found to be 100 and 67.18 %, respectively. Also, the sero-prevalence of H. contortus infection was found to be 58.66 % out of 150 migratory sheep serum samples screened for detecting circulating antibodies of H. contortus by Indirect-ELISA using somatic antigen in and around shimoga region.

In the present study, post-mortem was conducted on a female elephant aged about 37 years died at Rajeev Gandhi National Park, Hunsur, Mathigoodu Elephant Camp, Karnataka state. The animal suffered with diarrhoea, anorexia, dehydration and was unable to walk for about one week before death and was treated with antibiotics and fluid therapy for three days. The post-mortem examination revealed that, the gastric mucosa was severely congested, hyperaemic and numerous stomach bots attached to the mucosa. The bots were recovered from the gastric mucosa and processed for species identification. The posterior spiracles of the bots showed three longitudinal parallel slits in each spiracle, the abdominal segments had a row of belt like triangular shaped spines and the anterior end had two powerful oral hooks with cephalo-pharyngeal skeleton. Based on the above said morphological characters, the bots were identified as This seems to be the first report of in free range wild elephant from Karnataka state.

The checkered keelback is also known as Asiatic water snake and is common species of non-venomous snake known to occur in India. The present study report the occurrence of Ascaridia galli infection in a checkered keelback snake, which was found dead near Veterinary College hostel premises. The post-mortem examination of the snake revealed catarrhal exudate without ingesta in the stomach and showed gastric ulcerations with edema. Large numbers of round worms were packed in the lumen of small intestine with petechial haemorrhages and necrotic enteritis. The nematode parasites were recovered and processed for identification. Based on morphological characters, the worms were identified as A. galli and the intestinal contents of the snake showed A. galli eggs.

The present study was undertaken for the immuno-diagnosis of infection in sheep by counter immuno-electrophoresis (CIEP) using crude somatic antigen. Adult worms of were found in 57 out of 100 abomasums screened. A total of 250 serum samples which includes, 100 serum from local abattoir in and around Shimoga region from the sheep from which the abomasums were collected for worms and 150 serum from the migratory sheep were collected to detect the circulating antibody of by CIEP using crude whole worm somatic antigen. Out of 100 abomasums screened, 18 were heavily infected with adult worms of and their serum samples showed positive reaction with clear thick precipitin line with crude somatic antigen of by CIEP and 39 samples showed light precipitin line, which were moderately infected with adult worms of during necropsy. However, the 12 serum samples showed positive reaction with clear precipitin line, but their abomasums did not show and the remaining 31 were not harbouring any adult worms of and no precipitin line observed by CIEP. The sensitivity and specificity of CIEP was found 100 and 72.09 %, respectively. In the present study, 62 % sero-prevalence was observed with CIEP among 150 serum samples from migratory sheep screened for antibodies of using crude whole worm somatic antigen of