A -Relative expression of HSF-1 target genes following heat shock (33°C, 30 min) compared to control treated animals (20°C) on day 3 of adulthood. Values plotted are the mean of 4 biological replicates.B -Relative expression of HSF-1 target genes on day 3 of adulthood in wild type animals exposed to control conditions (20°C), 25°C for 1 hour or 28°C for 1 hour. Expression was calculated relative to the HK genes cdc-42, rpb-2 and pmp-3. Values plotted are the mean of 4 biological replicates. C -Representative images and fluorescence quantification of hsp-6p::gfp and hsp-16.2p::mcherry reporters on day 3 of adulthood following treatment with DMSO, antimycin A, rotenone or paraquat. Values plotted are the mean fluorescence of at least 15 worms per treatment group. D & E -Representative images and fluorescence quantification of day 3 adult wild type and (D) clk-1(qm30) or (E) glp-1(e2144ts) worms expressing hsp-16.2p::mcherry following exposure to empty vector (EV) control (L4440) or cox-6c(RNAi). Experiments involving glp-1 and clk-1 mutants were performed at 25°C and 20°C respectively. Values plotted are the mean fluorescence of at least 15 worms per treatment group. F-H -Representative images of (F) daf-16p::daf-16::GFP, (G) hsp-6p::gfp and (H) cyp14Ap::gfp worms following growth on empty vector (EV) control, cox-6c(RNAi) or EV/cox-6c(RNAi) combined with (F), daf-16(RNAi), (G) atfs-1(RNAi) or (H) nhr-45(RNAi). I -Fluorescence quantification of day 3 adult daf-16p::daf-16::GFP, hsp-6p::gfp and cyp14Ap::gfp worms. Values plotted are the mean fluorescence of at least 15 worms per treatment group. All scale bars = 250 µM. Statistical significance was calculated by: (B and C) one-way ANOVA with Tukey postanalysis comparison of groups and (D and E) two-way ANOVA with post analysis pairwise comparison of groups. All error bars represent SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

No abstract