The aim was to determine colonization rate of candida in oral cavity of healthy individuals, diabetics and HIV seropositive individuals. Material and methods: Samples were collected from oral cavity of 50 HIV sero positive individuals, 50 diabetics and 50 healthy individuals by swabbing palatal mucosa, dorsum of tongue and buccal mucosa with a sterile swab. Samples were processed by inoculating on Hi Chrome Agar and speciation was done by growth on Hi Chrome agar, germ tube test, chlamydospore formation on CMA, pellicle formation in SDA broth and growth at 45ºc. Results: 27 HIV sero positive individuals (54%) carried candida in their oral cavities (single strain in 44% and combination of strains in 10%). Whereas it was 44% in diabetics (single species in 38% and a combination of species in 6%) and 24% in healthy individuals (only single species). Candida albicans accounts for 41.66% in healthy individuals, 68% in diabetics and 42