Unani system of medicines classifies its formulation into several classes e. g. Huboob, Sufoof, Qurs, Sufoofs, Majoon, Arq, Roughan, Kushta etc. Among the various dosage forms of Unani system of medicine Sharbat Toot Siyah is one of the renowned formulations whichis widely used for the number of ailments e.g. Pharyngeal pain and sore throat. The drug Sharbat Toot Siyah was in-house manufactured in three batches of different volumes of 500, 600 and 700ml following the SOP guidelines and underwent for the evaluation of secondary metabolite constituents Profile with Physico-chemical Standardization of this important poly-herbal formulation. The present work contributes to the development of standardization parameters of herbal drugs used in Indian system of medicine like morphological properties, viscosity, pH values, ash values, extractive values, alcohol soluble matter, specific gravity at 25ºC, refractive index, sugar quantity e. g. total sugar, reducing sugar and non-reducing sugar, qualitative and quantitative inorganic elements, phyto-constituents and thin layer chromatography. In preliminary phytochemical investigation, analysis showed the presence of organic constituent’s amino acids, tannins, phyto-sterols, flavanoids and glycosides in the extract while in the elemental analysis, calcium, magnesium, phosphorous etc. were found to be present. The study also includes quality control parameters e. g. aflatoxins, microbial load, pesticide residue and detection of heavy metals which are helpful to ensure the purity, safety and efficacy of herbal formulation. The results so obtained for the various Physico-chemical tests may be taken as standard values for future reference.

Since the time Hippocrates man has made the use the different Unani medicines for the treatment of various ailments and still vast majority of population depends on these drugs. So the herbal drugs are gaining more and more attention all over the world due to their long historical clinical practice and less side effects. “Qurs- e- Zarishk” is one of the most important poly-pharmaceutical Unani formulation widely used for the treatment of liver disorders and anemia. Since the formulation has a very effective role in curing such diseases with zero side effects (unlike in Modern Medicine) so all the ingredients used in this preparation are required to be authenticated and standardized properly for quality control purposes. The present paper deals with different physicochemical and biological parameters like botanical identification, microbial load, aflatoxin, pesticide residue, determination of heavy metals, qualitative and quantitative analysis of phyto-chemical constituents, solubility in alcohol and water, successive extractions, pH values, ash values, quantitative estimation of total phenolics, resins etc. Besides, TLC studies of successive extracts have also been carried out.