BACKGROUND: Antibiotics have helped people to treat many diseases and have made the treatment of patients much easier. However, due to the development of resistance factors, antibiotics have become less effective against bacteria. Antimicrobial resistance is one of today's most important issues in global medicine. OBJECTIVE: to determine the expression of cephalosporin drug resistance in an inpatient setting. METHODS: This study was conducted at the Clinical Hospital of Emergency Medical Care Tver, Russia, for the period 2018-2020. Using the standard method of bacteriological examination, the microbiome of 624 patients was examined. The next step was to determine the level of antibiotic resistance. RESULTS: Microorganisms of different spectrum, including Pseudomonas aeruginosa, Escherichia coli, Acinetobacter, etc., were isolated from clinical samples during the study. All microorganism groups showed decreased susceptibility to the drugs used in the study. Ceftriaxone (a third-generation cephalosporin) had the greatest loss of potency (reduction to 0% for all isolates in the study). Cefoperazone sulbactam and ceftazidime rapidly lost activity in this study. Increasing resistance to cefepime (a fourth-generation cephalosporin) was observed. CONCLUSIONS. The study results indicate the rapid spread of resistance among a wide range of pathogenic microorganisms to the studied cephalosporin groups. Complete resistance developed within three years to the third-generation cephalosporins. A decrease in the susceptibility to the fourth generation of cephalosporins was observed.

Background: Antibiotic-resistance is one of the most important problems in modern medicine. The overuse and misuse of antibiotics caused the development of drug-resistant pathogens. It is the reason for more difficult treatment for patients. Objective: To monitor E.coli sensitivity to antibiotics and to evaluate its antibiotic resistance in a multidisciplinary hospital setting. Methods: The study is presented by a statistical analysis of the results of microbiological examination of materials obtained from patients infected with E.Coli, treated in different departments of Tver Clinical Emergency Hospital (Russia) in 2018-2020. A total of 142 results of microbiological examination of urogenital tract, abdominal cavity and wound surfaces were processed. Results: The number of antibiotics to which the Gram-negative Escherichia Coli is resistant also increases with the age of the patients. Among the investigated antibacterial drugs in terms of the total number of microorganisms with resistance to them, the lowest clinical efficacy was observed in the drugs of the cephalosporin series. Among the most frequent diseases caused by the studied strains of E.coli are intestinal infections and urinary tract infections. In addition, infections caused by persistence of E. coli as a background disease can significantly complicate the underlying disease. Conclusion: The results present a rapid spread of resistance genes in the studied E.coli strains. It is the imperative for the improvement of antibiotic resistance monitoring using various laboratory methods. Based on the results of microbiological research it is necessary to create a database that will allow to select the optimal strategy for the drug treatment of patients according to its resistance profile.