Environmental Literacy can empower individuals to make appropriate environment-related choices and act more responsibly towards the environment. Environmental literacy was measured among a sample of 461 pre-service teachers enrolled at the Department of Preschool Education at the University of Ioannina, in Greece. Results indicate that pre-service teachers have positive attitudes towards the environment, a moderate level of environmental knowledge while their participation in environmental actions is limited, particularly collective actions. Statistical analyses also highlighted that Environment Literacy depends on year of study and high school course specialization. These findings are further discussed.