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Road freight transportation is already contributing significantly to global warming, and its emissions are predicted to grow dramatically in the following years. Carbon footprint calculation can be used to assess CO2e emissions to understand how an organization’s activities impact global sustainability. To this end, the main objective of this paper is initially to assess the impact of Green House Gas (GHG) emissions stemming from road freight transportation. Subsequently, we adopt the EN 16258 standard to calculate the carbon footprint of a truck fleet of a freight transport operator in Greece. Based on the obtained results, we assess the performance of the company’s fleet by adopting relevant sustainability indicators. We also evaluate the use of CNG as an alternative fuel and its impact on CO2e emissions and operational costs. The paper concludes with a list of additional measures toward further reduction and offsetting of CO2e emissions.

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