TiVCr alloys known for their potentially large hydrogen uptake were formulated as (TiCr1.8)100-xVx in order to quantify the impact of vanadium concentration. Parallel to this series of pure ternary, a 2nd ones was synthesized with 4 at.% of Zr7Ni10 addition. Electrochemical charging with hydrogen reveals a fair dependence of hydrogen uptake and hydrides stability versus the x (V) content, moreover conversely different due to the presence of the Zr-Ni additive.

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The E3 ubiquitin-protein ligase HOS1 is an important integrator of temperature information and developmental processes. HOS1 is a negative regulator of plant cold tolerance, and silencing HOS1 leads to increased cold tolerance. In the present work, we studied ROS levels in hos1Cas9 Arabidopsis thaliana plants, in which the HOS1 gene was silenced by disruption of the open reading frame via CRISPR/Cas9 technology. Confocal imaging of intracellular reactive oxygen species (ROS) showed that the hos1 mutation moderately increased levels of ROS under both low and high light (HL) conditions, but wild-type (WT) and hos1Cas9 plants exhibited similar ROS levels in the dark. Visualization of single cells did not reveal differences in the intracellular distribution of ROS between WT and hos1Cas9 plants. The hos1Cas9 plants contained a high basal level of ascorbic acid, maintained a normal balance between reduced and oxidized glutathione (GSH and GSSG), and generated a strong antioxidant defense response against paraquat under HL conditions. Under cold exposure, the hos1 mutation decreased the ROS level and substantially increased the expression of the ascorbate peroxidase genes Apx1 and Apx2. When plants were pre-exposed to cold and further exposed to HL, the expression of the NADPH oxidase genes RbohD and RbohF was increased in the hos1Cas9 plants but not in WT plants. hos1-mediated changes in the level of ROS are cold-dependent and cold-independent, which implies different levels of regulation. Our data indicate that HOS1 is required to maintain ROS homeostasis not only under cold conditions, but also under conditions of both low and high light intensity. It is likely that HOS1 prevents the overinduction of defense mechanisms to balance growth.

TiVCr alloys known for their potentially large hydrogen uptake were formulated as (TiCr 1.8) 100-x V x in order to quantify the impact of the vanadium concentration. Parallel to this series of pure ternaries, a second one was synthesized with 4 mol.% of Zr 7 Ni 10 addition. Structural investigation and electrochemical charging with hydrogen revealed a fair dependence of the hydrogen uptake and hydride stability on the vanadium content, but conversely different due to the presence of the Zr-Ni additive.