The success of any industrial system hinges on the quality and effectiveness of its workers. The study which is non-experimental and cross-sectional survey examined work-family role interface conflict and organizational climate as correlates of teamwork effectiveness among industrial workers in Nnewi, South-East, Nigeria. Using a non-purposive sampling technique, a total number of two hundred and forty (240) industrial workers were selected from Chicason Groups of companies located at the commercial hub of Nnewi, Anambra State, Nigeria. The participants comprised of 143 (59.5%) males and 97 (40.5%) female industrial workers. Modified Teamwork Effectiveness Questionnaire (MTEQ), Work-Family Conflict and Family-Work Conflict Scale (WFC), and Organizational Climate Scale (CLIOR) were used to collect data from the participants. Pearson Product Moment Correlation (PPMC) was used to analyze the relationship between the variables of study while multiple regression was utilized to evaluate the independent and joint predictions of the predictor variables. Results of the multiple regression demonstrated that work-family role interface conflict negatively predicted teamwork effectiveness [β = -.24; p<.01] while perceived organizational climate showed significant positive prediction on teamwork effectiveness [β = .37; p<.01] among the industrial workers. Based on the findings of the study, it is recommended that organizational climate of the organization should be harnessed to the extent that it reflects reward for hardworking employees and create an atmosphere that make employees feel as an integral part of the organization while achieving the organizations goals and objectives.