Agroforestry systems (AFS) for cocoa production combine traditional land-use practices with local biodiversity conservation, resulting in both ecological and agricultural benefits. The cacao-cabruca AFS model is widely implemented in regions of the Brazilian Atlantic Forest. Carpotroche brasiliensis (Raddi) A. Gray (Achariaceae) is a tree found in cabruca landscapes that is often used for reforestation and biotechnological applications. Despite its importance, we still lack information about viruses circulating in C. brasiliensis, particularly considering the possibility of spillover that could affect cocoa production. In our study, we analyzed the Carpotroche brasiliensis virome from Atlantic Forest and cacao-cabruca AFS regions using metatranscriptomics from several vegetative and reproductive organs. Our results revealed a diverse virome detecting near-complete or partial coding sequences of single- and double-stranded DNA and RNA viruses classified into at least six families (Botourmiaviridae, Bromoviridae, Caulimoviridae, Genomoviridae, Mitoviridae, and Rhabdoviridae) plus unclassified elements. We described with high confidence the near-complete and the partial genomes of two tentative novel viruses: Carpotroche-associated ilarvirus and Carpotroche-associated genomovirus, respectively. Interestingly, we also described sequences likely derived from a rhabdovirus, which could represent a novel member of the genus Gammanucleorhabdovirus. We observed higher viral diversity in cacao-cabruca AFS and reproductive organs of C. brasiliensis with preferential tropism to fruits, which could directly affect production. Altogether, our results provide data to better understand the virome in this unexplored agroecological interface, such as cacao-cabruca AFS and forest ecosystem, providing information on the aspects of virus–plant interactions.

ResumoAs dorsalgias, conhecidas comumente como dores nas costas, podem ser provocadas por uma série de motivos. Algumas profissões exigem dos trabalhadores posturas inadequadas, que sobrecarregam a coluna vertebral e consequentemente podem causar dores. Devido à postura adotada na moto, o motoboy pode apresentar comprometimentos da coluna vertebral. Neste projeto buscou-se realizar um diagnóstico sobre as queixas de dores nas costas de mototaxistas do município de Itapetinga -Ba e desenvolver uma atividade de extensão, utilizando cartazes e vídeos informativos/educativos e conversas informais, passando informações, buscando a prevenção com a adoção de hábitos adequados à rotina de trabalho destes profissionais. Quase a metade dos mototaxistas do município de Itapetinga -Ba relata sentir dores nas costas diariamente, principalmente na região lombar. Esse dado aumenta quando relacionado à jornada de trabalho excessiva, inatividade física e maus hábitos posturais. Esse tipo de problema pode ser evitado ou minimizado com mudanças de hábitos. As atividades de extensão surtem efeito positivo, nesta perspectiva preventiva, podendo trazer maior qualidade já que são bem aceitos pela população. Palavras-chave: Coluna vertebral. Mototaxistas. Prevenção. AbstractA number of reasons can trigger Dorsalgias, commonly known as back pain. Some professions require workers to pose inappropriate postures, which overload the spine and consequently can cause pain. Due to the stance taken on the bike, the motorcycle may have spinal compromises. In this project, a diagnosis was made on the complaints of back pain of motorcycle taxi driver of the municipality of Itapetinga-Ba and to develop an extension activity, using informative / educational posters and videos and informal conversations, passing information aiming at prevention with the adoption of habits appropriate to the work routine of these professionals. Almost half of the