During adult life, honey bee workers undergo a succession of behavioral states. Nurse bees perform tasks inside the nest, and when they are about 2-3 weeks old they initiate foraging. This switch is associated with alterations in diet, and with the levels of juvenile hormone and vitellogenin circulating in hemolymph. It is not clear whether this behavioral maturation involves major changes at the cellular level, such as mitochondrial activity and the redox environment in the head, thorax and abdomen. Using high-resolution respirometry, biochemical assays and RT-qPCR, we evaluated the association of these parameters with this behavioral change. We found that tissues from the head and abdomen of nurses have a higher oxidative phosphorylation capacity than those of foragers, while for the thorax we found the opposite situation. As higher mitochondrial activity tends to generate more HO, and HO is known to stabilize HIF-1α, this would be expected to stimulate hypoxia signaling. The positive correlation that we observed between mitochondrial activity and gene expression in abdomen and head tissue of nurses would be in line with this hypothesis. Higher expression of antioxidant enzyme genes was observed in foragers, which could explain their low levels of protein carbonylation. No alterations were seen in nitric oxide (NO) levels, suggesting that NO signaling is unlikely to be involved in behavioral maturation. We conclude that the behavioral change seen in honey bee workers is reflected in differential mitochondrial activities and redox parameters, and we consider that this can provide insights into the underlying aging process.

ReSUmOO estudo realizado aborda a problemática envolvendo os resíduos sólidos de serviços de saúde e a atitude do enfermeiro perante os aspectos relacionados ao gerenciamento e a conscientização ambiental. Participaram da pesquisa dez profissionais atuantes na Estratégia de Saúde da Família, dos municípios de Araputanga, Mirassol D'oeste e São José dos Quatro Marcos. Os dados foram obtidos através da aplicação de questionários, posteriormente analisados através do software Origin ® . Observou-se o nível de conhecimento em relação à legislação vigente, etapas de manejo realizadas em âmbito municipal e capacitação da equipe e, ainda, a respeito da habilidade dos profissionais em diagnosticar situações de risco ocupacional e para a saúde pública. Apesar da existência de um significativo conhecimento sobre o tema, ainda há necessidade de trabalhos de conscientização e desenvolvimento de práticas adequadas de gerenciamento de Resíduos de Serviços de Saúde. descritores: Enfermagem em Saúde Pública; Resíduos de Serviços de Saúde; Educação em Saúde. aBStRaCt The study addresses the problem involving the solid waste from health service and the nurses attitude towards aspects related to the management and environmental awareness. Participants were ten professionals working in the Family Health Strategy, from the municipalities of Araputanga, Mirassol D'west and São José dos Quatro Marcos. Data were collected through questionnaires, and analyzed using the software Origin ® . It was observed the level of knowledge regarding the current law, management steps taken at the municipal and staff training, and also about the ability of the professionals to diagnose situations and occupational risk to public health. Despite the existence of a significant knowledge on the subject, there is still need to work on awareness and development of appropriate management practices on the health services waste. Key words: Public Health Nursing; Wastes of Health Services; Health Education. ReSUmeNEl estudio aborda los problemas relacionados con los residuos sólidos del servicio de salud y la actitud de los enfermeros hacia los aspectos relacionados con la gestión y la concientización ambiental. Participaran del estudio diez profesionales que trabajan en la Estrategia de Salud Familiar, en los municipios de Araputanga, Mirassol D'Oeste y São José dos Quatro Marcos. Los datos se obtuvieron mediante la aplicación de cuestionarios, posteriormente analizados a través del software Origin ® . Se observó el nivel de conocimientos sobre la legislación vigente, las medidas de gestión adoptadas en ámbito municipal y también la capacidad de los profesionales para diagnosticar situaciones de riesgos ocupacionales y para la salud pública. A pesar de la existencia de un conocimiento significativo sobre el tema, todavía hay que trabajar en la sensibilización y en el desarrollo de prácticas apropiadas de gestión de los residuos de los servicios de salud Palabras clave:

Background: Exercise training may improve energy expenditure, thermogenesis, and oxidative capacities. Therefore, we hypothesized that physical training enhances white adipose tissue mitochondrial oxidative capacity from obese women. Objective: To evaluate mitochondrial respiratory capacity, mitochondrial content, and UCP1 gene expression in white adipose tissue from women with obesity before and after the physical training program. Methods: Women (n = 14, BMI 33 ± 3 kg/m 2 , 35 ± 6 years, mean ± SD) were submitted to strength and aerobic exercises (75%-90% maximum heart rate and multiple repetitions), 3 times/week during 8 weeks. All evaluated subjects were paired, before and after training for resting metabolic rate (RMR), substrate oxidation (lipid and carbohydrate) by indirect calorimeter, deuterium oxide body composition, and aerobic maximum velocity (V max ) test. At the beginning and at the ending of the protocol, abdominal subcutaneous adipose tissue was collected to measure the mitochondrial respiration by high-resolution respirometry, mitochondrial content by citrate synthase (CS) activity, and UCP1 gene expression by RT-qPCR. Results: Combined physical training increased RMR, lipid oxidation, and V max but did not change body weight/composition. In WAT, exercise increased CS activity, decreased mitochondrial uncoupled respiration and mRNA of UCP1. RMR was positively correlated with fat-free mass. Conclusion: Physical training promotes an increase in mitochondrial content without changing tissue respiratory capacity, a reduction in mitochondrial uncoupling degree and UCP1 mRNA expression in WAT. Finally, it improved the resting metabolic rate, lipid oxidation and physical performance, independent of the body changing free, or fat mass in obese women. K E Y W O R D Scitrate synthase, human energy metabolism, mitochondria, obesity, physical training, uncoupling protein 1, white adipose tissue

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