The study aimed to determine the secondary metabolites contained in raw banana peel waste, and to test the inhibitory activity in bacteria. Banana peel waste is used namely; kepok banana (Musa acuminata x balbisiana), uli banana (Musa paradisiaca sapientum) and nangka banana (Musa sp L) extracted using distilled water with the dekok method. The phytochemicals and antibacterial activity were tested for Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Phytochemical test results showed the three banana peel extracts contain the same compounds, namely alkaloids, flavonoids, saponins, tannins and phenols. Different content of kepok banana peel extract is triterpenoid compounds which are not possessed by uli banana peel extract or nangka banana extract. The result shows that the higher concentration of extract used, the greater an inhibitory power of the bacteria. While, antibacterial test results showed that 25% concentration of uli banana peel had the best antibacterial inhibition on S. aureus bacteria compared with kepok banana peel extract and nangka banana could not inhibit the growth of E. coli bacteria.
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